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Team:TU-Munich/Notebook/Materials
From 2013.igem.org
(Difference between revisions)
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|TEV_fw | |TEV_fw | ||
| - | |<code>5'- | + | |<code>5'- gcgcgtctagatggccggcggagaaagcttgtttaaggggc - 3'</code><br> |
|- | |- | ||
| | | | ||
|TEV_rv | |TEV_rv | ||
| - | |<code>5'- | + | |<code>5'- atataaccggtattcatgagttgagtcgcttcc - 3'</code><br> |
|- | |- | ||
| | | | ||
|TEV_t387c_fw | |TEV_t387c_fw | ||
| - | |<code>5'- | + | |<code>5'- atggtgtcagacactagctgcacattcccttcatc - 3'</code><br> |
|- | |- | ||
| | | | ||
|TEV_t387c_rv | |TEV_t387c_rv | ||
| - | |<code>5'- | + | |<code>5'- gatgaagggaatgtgcagctagtgtctgacaccat - 3'</code><br> |
|- | |- | ||
| | | | ||
|N_split_TEV_fw | |N_split_TEV_fw | ||
| - | |<code>5'- | + | |<code>5'- attcgcggccgcttctag - 3'</code><br> |
|- | |- | ||
| | | | ||
|N_split_TEV_rv | |N_split_TEV_rv | ||
| - | |<code>5'- | + | |<code>5'- ttcgaaaccggtagtttggaagttggttgtcac - 3'</code><br> |
|- | |- | ||
| | | | ||
|C_split_TEV_fw | |C_split_TEV_fw | ||
| - | |<code>5'- | + | |<code>5'- aagctttctagatggccggcaagagcatgtctagcatggtg - 3'</code><br> |
|- | |- | ||
| | | | ||
| - | | | + | |C_split_TEV_rv |
| - | |<code>5'- | + | |<code>5'- ccgctactagtattaaccgg - 3'</code><br> |
|- | |- | ||
| | | | ||
|PIF6_2-100_fw | |PIF6_2-100_fw | ||
| - | |<code>5'- | + | |<code>5'- aagctttctagatggccggcatgttcttaccaaccgattattgttgc - 3'</code><br> |
|- | |- | ||
| | | | ||
|PIF6_2-100_rv | |PIF6_2-100_rv | ||
| - | |<code>5'- | + | |<code>5'- ttcgaaaccggtgtcaacatgtttattgctttc - 3'</code><br> |
|- | |- | ||
| | | | ||
Revision as of 13:48, 7 May 2013
Oligonucleotides
| Name | Sequence | |
| EreA_for | 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘ | |
| EreA-rev | 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘ | |
| EreA_SDMI_for | 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘ | |
| EreA_SDMI_rev | 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3' | |
| EreA_SDMII_for | 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3' | |
| EreA_SDMII_rev | 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3' | |
| EreA_SDMIII_for | 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3' | |
| EreA_SDMIII_rev | 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3' | |
| EreB_for | 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3' | |
| EreB-rev | 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3' | |
| EreB_SDMI_for | 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3' | |
| EreB_SDMI_rev | 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3' | |
| EreB_SDMII_for | 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3' | |
| EreB_SDMII_rev | 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3' | |
| EreB_SDMIII_for | 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3' | |
| EreB_SDMIII_rev | 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3' | |
| TEV_fw | 5'- gcgcgtctagatggccggcggagaaagcttgtttaaggggc - 3' | |
| TEV_rv | 5'- atataaccggtattcatgagttgagtcgcttcc - 3' | |
| TEV_t387c_fw | 5'- atggtgtcagacactagctgcacattcccttcatc - 3' | |
| TEV_t387c_rv | 5'- gatgaagggaatgtgcagctagtgtctgacaccat - 3' | |
| N_split_TEV_fw | 5'- attcgcggccgcttctag - 3' | |
| N_split_TEV_rv | 5'- ttcgaaaccggtagtttggaagttggttgtcac - 3' | |
| C_split_TEV_fw | 5'- aagctttctagatggccggcaagagcatgtctagcatggtg - 3' | |
| C_split_TEV_rv | 5'- ccgctactagtattaaccgg - 3' | |
| PIF6_2-100_fw | 5'- aagctttctagatggccggcatgttcttaccaaccgattattgttgc - 3' | |
| PIF6_2-100_rv | 5'- ttcgaaaccggtgtcaacatgtttattgctttc - 3' | |
| MinMCS_fo | 5'- ctagacctaggaagcttcctgcaggactagtaagcttctgca - 3' | |
| MinMCS_rev | 5'- gaagcttactagtcctgcaggaagcttcctaggt - 3' | |
| Bpu_for | 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3' | |
| Bpu_rev | 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3' | |
| Bpu_SDMI_for | 5'- cacttaagcattttctacgggtcgatcacaccattca - 3' | |
| Bpu_SDMI_rev | 5'- tgaatggtgtgatcgacccgtagaaaatgcttaagtg - 3' | |
| Bpu_SDMII_fo | 5'- gacacgattaaatgtgtatgcgggcttagctggattttatttg - 3' | |
| Bpu_SDMII_re | 5'- caaataaaatccagctaagcccgcatacacatttaatcgtgtc - 3' | |
| Bpu_SDMIII_fo | 5'- cacccttaaaaataaagctggctgcggacaggaag - 3' | |
| Bpu_SDMIII_re | 5'- cttcctgtccgcagccagctttatttttaagggtg - 3' | |
| Bpu_SDMIV_fo | 5'- cgtacgctcactcttacgggtacacaggataaata - 3' | |
| Bpu_SDMIV_re | 5'- tatttatcctgtgtacccgtaagagtgagcgtacg - 3' | |
| xylE_for | 5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3' | |
| xylE_rev | 5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3' | |
| xylE_c312a_for | 5'- GAACAGTTGTGGACGGCGCGTGCGC - 3' | |
| xylE_c312a_rev | 5'- GCGCACGCGCCGTCCACAACTGTTC - 3' | |
| xylE_g483a_for | 5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3' | |
| xylE_g483a_rev | 5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3' | |
| xylE_a834g_for | 5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3' | |
| xylE_a834_rev | 5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3' | |
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' |
