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Team:UTK-Knoxville/6 June 2013
From 2013.igem.org
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<p>Ivan, Brandon, Eric, and Patrick met to discuss the RcoM, HemAT, and cytoplasmic primers and the wiki page.</p> | <p>Ivan, Brandon, Eric, and Patrick met to discuss the RcoM, HemAT, and cytoplasmic primers and the wiki page.</p> | ||
<p>Ligation Aer/EnvZ + OmpR + BB</p> | <p>Ligation Aer/EnvZ + OmpR + BB</p> | ||
Latest revision as of 20:53, 30 July 2013
June 6th 2013
Ivan, Brandon, Eric, and Patrick met to discuss the RcoM, HemAT, and cytoplasmic primers and the wiki page.
Ligation Aer/EnvZ + OmpR + BB
3:1 - 1 uL BB, 1 uL Ligase, 1 uL Buffer, 1.75 uL OmpR, 1.75 uL Aer/EnvZ, 3.5 uL sterile DI water.
5:1 - 1 uL BB, 1 uL Ligase, 1 uL Buffer, 3 uL OmpR, 3 uL Aer/EnvZ, 1 uL sterile DI water.
Room Temperature for 2 hours.
We received the strains with OmpR and EnvZ individually knocked out. Brandon grew these up in liquid media and put the plates in the 4 C room. We are still waiting to grow up ΔOmpR + ΔEnvZ strains.
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