Team:Evry/Protocols/04

From 2013.igem.org

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<h2> LB and LB Agar </h2>
<h2> LB and LB Agar </h2>
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Put 20 g of LB Broth  and pour 1L of distilled water in a bottle of 1L.<br>
+
Mix 20 g of LB Broth  in 1L of distilled water in a bottle of 1L. Put in autoclave overnight. <br>
-
Put 17,5 g of LB Agar and pour 500 mL of distilled water in a bottle of 1L.
+
Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L. Put in autoclave overnight.
 +
<br><br>
<h2> CaCl2 and CaCl2+glycerol </h2>
<h2> CaCl2 and CaCl2+glycerol </h2>

Revision as of 11:28, 31 July 2013

Solutions, Media and Petri Dishes

Antibiotics

LB and LB Agar

Mix 20 g of LB Broth in 1L of distilled water in a bottle of 1L. Put in autoclave overnight.
Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L. Put in autoclave overnight.

CaCl2 and CaCl2+glycerol

Tris HCl