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	Adjust pH with concentrated HCl(~4 mL) until pH=7,5.<br>		Adjust pH with concentrated HCl(~4 mL) until pH=7,5.<br>
	Add distilled water until 50 mL then put in autoclave.<br>		Add distilled water until 50 mL then put in autoclave.<br>
-	If the solution has a yellow coloration, do the preparation again with better Trisbase.	+	If the solution has a yellow coloration, do the preparation again with better Trisbase.<br>
	Tris HCl is used to store DNA.		Tris HCl is used to store DNA.
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Revision as of 11:47, 31 July 2013

Solutions, Media and Petri Dishes

Antibiotics

LB and LB Agar

Mix 20 g of LB Broth in 1L of distilled water in a bottle of 1L, then put in autoclave.
Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L, then put in autoclave.

CaCl2 and CaCl2+glycerol

Tris HCl

Disolve 4,6g of Trisbase in 30 mL of distilled water.
Adjust pH with concentrated HCl(~4 mL) until pH=7,5.
Add distilled water until 50 mL then put in autoclave.
If the solution has a yellow coloration, do the preparation again with better Trisbase.
Tris HCl is used to store DNA.