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Team:MSOE Milwaukee/Week9
From 2013.igem.org
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<H1 align = left>Thursday</H1> | <H1 align = left>Thursday</H1> | ||
| - | <p> </p><br> | + | <p>The competent cell protocol was carried out in lab. It seemed to go as planned, but we will not know if it worked for sure until Friday. We also found out that our order is coming in sometime on Monday, so we will for sure be able to begin our lab work next week. </p><br> |
<H1 align = left>Friday</H1> | <H1 align = left>Friday</H1> | ||
| - | <p></p> | + | <p>The competent cell protocol was a sucess! We checked the growth of the cells, and we had plenty of colonies. We plan to use these cells next week once our parts arrive. </p> |
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{{Team:MSOE:Notebook}} | {{Team:MSOE:Notebook}} | ||
Revision as of 01:21, 5 August 2013
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Week 9
Monday
As a team, we completed the protocol binder and want to double check our work on it. We asked our advisor some final questions on our procedures and determined that we are ready to get into lab. We have a few items left to buy and see if we have them currently in our lab, but we hope to get started in lab late this week, but for sure by early next week.
Tuesday
We started to map out our output plasmids using a free download of ApE software. We had to put the sequences in and it would allow us to label different parts of our plasmid. We hope to use these images on the website once they're completed. We also completed a few miscellaneous tasks.
Wednesday
We signed paperwork to gain lab access and learned more about the labs we will be using. We unpacked boxes of stuff we are allowed to use into the cabinet and got organized. We labeled all of our shelves and took inventory of our stocks. We found out that we didn't have one component for our previously found competent cell protocol, so we found a new protocol online and began day one of that one.
Thursday
The competent cell protocol was carried out in lab. It seemed to go as planned, but we will not know if it worked for sure until Friday. We also found out that our order is coming in sometime on Monday, so we will for sure be able to begin our lab work next week.
Friday
The competent cell protocol was a sucess! We checked the growth of the cells, and we had plenty of colonies. We plan to use these cells next week once our parts arrive.
