Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period3/Dailylog

From 2013.igem.org

(Difference between revisions)

Revision as of 02:15, 5 August 2013

Small Phage July - August Notebook: August 1 - August 16 Daily Log

8/1/13

- Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.

8/2/13

- Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration.

- Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration.

- Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Experiment 1.

8/3/13

- E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. Specifically, we added 1mL of LB three different test tubes.

test tube 1: used a pipet tip to stab the agar stock, after which pipet up and down to get the agar into LB
test tube 2: wooden stick to scrap the agar stock, after which submerge it in LB
test tube 3: after preparing test tube 2, the wooden stick was submerged directly in LB in test tube 3

7/22/13

- A lot of ousekeeping:

Went over parental guide, and pre-/post- questions for The Adventure of Baxtor Bacteria

Discussed next steps

Cleanned out fridge and took pictures of plates

Updated website

7/26/13

- Designed protocols for 7.29 Mutagen Concentration Test - Sixth Protocol and decided to conduct an experiment to verify bacteria growth curve (specifics see 7.27 Bacteria Growth Curve After Dilution)

- Started 3mL of BL21 liquid culture overnight.

7/27/13

- Conducted 7.27 Bacteria Growth Curve After Dilution

7/28/13

- Started 15mL of BL21 liquid culture overnight.

- Started phage liquid culture to test for superinfection.

7/29/13

- Performed part 1 of 7.29 Mutagen Concentration Test - Sixth Protocol.

- Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.)

7/31/13

- Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in 7.29 Mutagen Concentration Test - Sixth Protocol.

@@ Line 44: / Line 44: @@
 - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration.
-- Streaked E coli W3110 and K12 from frozen stock.
+- Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in  [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Experiment 1]].
 <br>