06/08/13

From 2013.igem.org

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(→Running of an agarose gel)
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!align="center"|[[Team:Leicester|Home]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
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!align="center"|[[Team:Leicester/Project|Project]]
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!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Leicester/Modeling|Modeling]]
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!align="center"|[[Team:Leicester/Notebook|Notebook]]
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==Running of an agarose gel==
==Running of an agarose gel==
*Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
*Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.

Revision as of 12:32, 6 August 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Running of an agarose gel

  • Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.

Making chloroamphenicol agar plates

  • Making additional plates
    • 400ml of agar
      • Melt in microwave
      • 3mins on low -> shake
      • Repeat
      • 2mins on low -> shake
      • Repeat
    • Add 800ul of chloroamphenicol at a concentration of 25 ul/ml
    • Pour out onto 20 petri dishes
    • Leave to set on bench

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