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07/08/13
From 2013.igem.org
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AliceHaworth (Talk | contribs) |
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|3 5/45||0.1||0.24||-0.29 | |3 5/45||0.1||0.24||-0.29 | ||
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| - | |Overnight | + | |Overnight* |
|- | |- | ||
|1in 10||24.8||1.8||2.42 | |1in 10||24.8||1.8||2.42 | ||
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*concentration of DNA was measured using nanodrop. | *concentration of DNA was measured using nanodrop. | ||
| - | *Dilutions | + | *Dilutions left on the bench overnight. |
== Gel electrophoresis of Dilluted Herring sperm DNA== | == Gel electrophoresis of Dilluted Herring sperm DNA== | ||
Revision as of 13:50, 7 August 2013
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Dillution of Herring sperm DNA
| Sample | Concentration (ng/ul) | 260/280 | 260/230 |
| 1kb | 534 | 1.82 | 1.95 |
| 1 5/45 | 52.7 | 1.77 | 2.05 |
| 2 5/45 | 4.6 | 1.46 | 1.88 |
| 3 5/45 | 0.1 | 0.24 | -0.29 |
| Overnight* | |||
| 1in 10 | 24.8 | 1.8 | 2.42 |
| 1 | 2.1 | 1.67 | 22.44 |
| 2 | 0.8 | 2.87 | -3.99 |
| Fresh stock | |||
| 1s | 5341.8 | 1.78 | 2.15 |
| 2s | 440.0 | 1.80 | 2.23 |
| 3s | 45.1 | 1.83 | 2.26 |
- concentration of DNA was measured using nanodrop.
*Dilutions left on the bench overnight.
Gel electrophoresis of Dilluted Herring sperm DNA
Lanes are: marker, 1 5/45, 2 5/45, 3 5/45, 1, 2, 3, 1s, 2s, 3s, lambda NT, lambda
