Team:DTU-Denmark/Notebook/6 July 2013

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==Procedure==
==Procedure==
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Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html| link]). Used smaller amount though.
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Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though.
Then, viewed the periplasmic and cytoplasmic cell contents under UV light.
Then, viewed the periplasmic and cytoplasmic cell contents under UV light.

Revision as of 08:50, 16 August 2013

6_July_2013


Contents

208 lab


Main purposes today


Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.

Who was in the lab


Kristian, Helen

Procedure


Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though.

Then, viewed the periplasmic and cytoplasmic cell contents under UV light.

Conclusion from today


The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.

DTU-Denmark HelloWorld Sucrose A+D+C 2.jpg


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