Team:KAIT Japan/Results

From 2013.igem.org

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='''Results'''=
='''Results'''=
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1.IL10recepter
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We inserted Il10receptere gene into vecter
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1 transformation
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Figure 1transformation of Il10recepter α
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Figure2 transformation of Il10recepter β
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2 .culture on the LB nutrient medium and get colonies.
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3 colony PCR
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The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp
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Figure3 Il10recepter α colony PCR
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Figure4 Il10recepter β colony PCR
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4 restriction enzyme processing PCR production
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5 infusion
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6 .transformation
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7 culture on the LB nutrient medium and get colonies.
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8 colony PCR
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The molecular weight of the vector is a 4000 ~ 5000bp.
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We were not able to confirm the vector by colony PCR.
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2 STAT3
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We inserted stat3 gene into vector
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1 transformation
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Figure5 transformation of STAT3
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2 .culture on the LB nutrient medium and get colonies.
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3 colony PCR
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The molecular weight of the α chain is 2307bp.
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Figure6 STAT3 colony PCR
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4 restriction enzyme processing PCR production
 +
5 infusion
 +
6 .transformation
 +
7 culture on the LB nutrient medium and get colonies.
 +
8 colony PCR
 +
The molecular weight of the vector is a 4000 ~ 5000bp.
 +
We were not able to confirm the vector by colony PCR.
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3 GFP+HlyA
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We inserted GFP gene and HlyA into vecter
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1 transformation
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Figure 1transformation of GFP.
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Figure2 transformation of HlyA.
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2 .culture on the LB nutrient medium and get colonies.
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3 colony PCR
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The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp
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Figure3 Il10recepter GFP PCR
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Figure4 Il10recepter HlyA PCR
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4 restriction enzyme processing PCR production
 +
5 infusion
 +
6 .transformation
 +
7 culture on the LB nutrient medium and get colonies.
 +
8 colony PCR
 +
The molecular weight of the vector is a 2000~3000bp.
 +
We were not able to confirm the vector by colony PCR.
 +

Revision as of 01:40, 28 September 2013

KAIT Japan 2013RogoTop.png

KAIT Japan2013 Kanako.png KAIT Japan 2013 iGEMRogo.png

KAIT Japan home.png Home
KAIT Japan team.png Team
KAIT Japan project.png Project
KAIT Japan parts.png Parts
KAIT Japan protocol.png Protocol
KAIT Japan notebook.png Notebook

KAIT_Japan_results.png Results

KAIT Japan safety.png Safety
KAIT Japan human practice.png Human Practice


Results

1.IL10recepter We inserted Il10receptere gene into vecter

1 transformation Figure 1transformation of Il10recepter α Figure2 transformation of Il10recepter β 2 .culture on the LB nutrient medium and get colonies. 3 colony PCR The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp Figure3 Il10recepter α colony PCR Figure4 Il10recepter β colony PCR 4 restriction enzyme processing PCR production 5 infusion 6 .transformation 7 culture on the LB nutrient medium and get colonies. 8 colony PCR The molecular weight of the vector is a 4000 ~ 5000bp. We were not able to confirm the vector by colony PCR.

2 STAT3 We inserted stat3 gene into vector 1 transformation Figure5 transformation of STAT3 2 .culture on the LB nutrient medium and get colonies. 3 colony PCR The molecular weight of the α chain is 2307bp. Figure6 STAT3 colony PCR 4 restriction enzyme processing PCR production 5 infusion 6 .transformation 7 culture on the LB nutrient medium and get colonies. 8 colony PCR The molecular weight of the vector is a 4000 ~ 5000bp. We were not able to confirm the vector by colony PCR.

3 GFP+HlyA We inserted GFP gene and HlyA into vecter

1 transformation Figure 1transformation of GFP. Figure2 transformation of HlyA. 2 .culture on the LB nutrient medium and get colonies. 3 colony PCR The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp Figure3 Il10recepter GFP PCR Figure4 Il10recepter HlyA PCR 4 restriction enzyme processing PCR production 5 infusion 6 .transformation 7 culture on the LB nutrient medium and get colonies. 8 colony PCR The molecular weight of the vector is a 2000~3000bp. We were not able to confirm the vector by colony PCR.






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