29/08/13

From 2013.igem.org

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{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
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!align="center"|[[Team:Leicester|Home]]
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!align="center"|[[Team:Leicester/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
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!align="center"|[[Team:Leicester/Project|Project]]
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!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Leicester/Modeling|Modeling]]
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!align="center"|[[Team:Leicester/Notebook|Notebook]]
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!align="center"|[[Team:Leicester/Safety|Safety]]
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!align="center"|[[Team:Leicester/Attributions|Attributions]]
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==Make 2% agarose gel==
==Make 2% agarose gel==
==Run Tod operon ligation genes on the gel==
==Run Tod operon ligation genes on the gel==

Latest revision as of 11:29, 2 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Contents

Make 2% agarose gel

Run Tod operon ligation genes on the gel

  • 1.5h at 70V

Isolate the DNA from gel

  • DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol

Measuring concentration of DNA using nanodrop

SampleVolume ulConcentration ng/ul260/280260/230
A13014.71.811.41
A231131.711.43
B13023.61.931.02
B22921.91.881.33
C12914.71.830.63
C23015.51.860.64
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