"
Team:BYU Provo/Notebook/Phage Purification/Springexp/Period2/Dailylog
From 2013.igem.org
| Line 32: | Line 32: | ||
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog" style="display:block;float:right;">Next >></a> | <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog" style="display:block;float:right;">Next >></a> | ||
</html> | </html> | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <font size="4"> '''5/13/13''' </font> | ||
| + | |||
| + | - Thankfully we don’t have to use the ultracentrifuge to achieve 10,000 g. We also won’t need to order any special centrifuge tubes, so we should be able to start experimentation on Wednesday. | ||
| + | |||
| + | -We added 1 mL of w3110 and 1 mL of BL21 to 24 mL of LB each and placed them both into the 37C incubator. After an hour of incubation we infected the BL21 Ecoli with t7 phage and left to incubate until wednesday. We will infect the w3110 tomorrow with T4 | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <font size="4"> '''5/14/13''' </font> | ||
| + | |||
| + | - Today we came in and infected 24 mL of LB with 1 mL of w3310 and put in the 37C incubator overnight. | ||
| + | |||
| + | I also infected 25 mL of w3110 with 100 µL of t4 phage and left overnight in the 37C incubator overnight. | ||
| + | |||
<br> | <br> | ||
Revision as of 18:50, 6 September 2013
| | ||
|
|
5/13/13 - Thankfully we don’t have to use the ultracentrifuge to achieve 10,000 g. We also won’t need to order any special centrifuge tubes, so we should be able to start experimentation on Wednesday. -We added 1 mL of w3110 and 1 mL of BL21 to 24 mL of LB each and placed them both into the 37C incubator. After an hour of incubation we infected the BL21 Ecoli with t7 phage and left to incubate until wednesday. We will infect the w3110 tomorrow with T4
5/14/13 - Today we came in and infected 24 mL of LB with 1 mL of w3310 and put in the 37C incubator overnight. I also infected 25 mL of w3110 with 100 µL of t4 phage and left overnight in the 37C incubator overnight.
5/20/13 - Performed a titer on both T4 and T7 to see if purification was successful.
5/22/13 - Repeated T4 titer as the last titer showed contamination. - Created a new 25 mL stock of BL21 E. coli
5/24/13 - Ran a CsCl gradient to further purify phage from
5/26/13 - Began the PEG purification.
| |
