Team:Uppsala/resveratrol-group

From 2013.igem.org

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  <h1>Methods</h1>
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  Tyrosine ammonia lyase (TAL) from rhodobacter sphaeroides was obtained from J.Conrado et al<sup><a>[1]</a></sup>
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  We biobricked TAL with the ribosome binding site B0034 and overhangs in a single pcr. We also made a version with 6-HIS-tag for enzyme expression analysis.
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  TAL was also mutagenized to remove illegal Not1 site. We verify all of our genetical constructs with sequencing.
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  We have expressed TAL in e-coli DH5alpha and E-coli nissle, a probiotic e-coli obtained from Trieste iGEM 2012. TAL will also be characterized in lactobacillus,
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  by transforming the construct with our shuttle vector.
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  This construct can also be used to produce the precursor for example our resveratrol.
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  <img class="hej" src="https://static.igem.org/mediawiki/2013/9/9a/Uppsala_TAL.png">
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  <img class="hej" src="https://static.igem.org/mediawiki/2013/d/d1/Uppsala_4CL-STS.png">
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Revision as of 09:22, 9 September 2013

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