Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer

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Phage Purification September - August Notebook: Experiments

9.7 CsCl Gradient

I) Purpose

Create a 10X solution of Phage Suspension Buffer that will be used to run our Phage Purification Centrifugation.

II) Expected Outcome

We should have a working phage suspension buffer that will facilitate movement of phage in the CsCl gradient.

III) Reagants Used

Tris-HCl

MgCl2

NaCl

Ultrapure H20

NaOH

IV) Actual Procedure

Add 58.44 g of NaCl to a 1 Liter Flask to achieve a 1 M solution.

Add 20.33 g of MgCl2 to the same 1 Liter Flask to achieve a .1 M solution.

Add 100 mL of 1 M concentration of tris-HCl 6.8 ph to the same flask.

Fill the flask with ultrapure H2O until it reaches the 1 L mark.

With a pH reader read the pH of the solution.

Add 5 microliters of NaOH at a time until the solution reads 7.2-7.5 pH on the pH meter.

V) Results