Team:BYU Provo/Notebook/Phage Purification/Springexp

(Difference between revisions)

Revision as of 22:09, 7 June 2013

Phage Purification May - June Notebook
Overview March-April May-June July-August September-October	May 1 - May 12 This semester we will are perfecting our procedures of purifying T4 and T7 bacteriophage through use of PEG and a CsCl gradient. Daily log Experiment Listing Progress Report
	May 13 - May 26 In these two weeks, we performed our first round of mutagenesis and first PCR. Although we are still testing the results to optimize our protocol, we are optimistic about the outcome! Daily log Experiment Listing Progress Report
	May 27 - June 9 We finished our CsCl gradient and have started a new round of phage purification to run through the CsCl gradient again. Daily log Experiment Listing Progress Report
	June 10 - June 23 Add description! Daily log Experiment Listing Progress Report
	June 24 - June 30 Add description! Daily log Experiment Listing Progress Report

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-<font size="3" font face="Calibri"> This is the start of Spring Term at BYU! The weather is finally getting better: no more snowing. And we are ready to do some serious lab work! While we are waiting for our mutgen, 5-bromodeoxyuridine, to arrive, we decided to start this term with establishing the basic procedures for our direct evolution method. Specifically, this involves making sure that we can (1) grow and amplify T7 phage in liquid culture, (2) select for smaller phage using concentrated top agar, and (3) amplify phage from a single plaque. During these two weeks, we also designed primers for cloning and sequencing T7 capsid proteins as well as mapped out detailed procedures for both cloning T7 capsid proteins and applying mutagen. </font>
+<font size="3" font face="Calibri"> This semester we will are perfecting our procedures of purifying T4 and T7 bacteriophage through use of PEG and a CsCl gradient. </font>
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