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Revision as of 23:39, 7 June 2013

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• iGEM 2013

Phage Purification May - June Notebook: May 13 - May 26 Daily Log

Overview March-April May-June July-August September-October

5/29/13 - Finished the PEG purification. 5.26 PEG Purification 5/31/13 - Worked on setting up our notebook online. 6/3/13 - Ran the CsCl purification step. 6.3 CsCl Gradient Phage Purification - Next class we will attempt extracting the phage from the gradient. 6/5/13 - Extracted the phage from 6.3 CsCl Gradient Phage Purification using a syringe and a needle. Only extracted T4 because the T7 gradient was destroyed in the refrigerator. Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed. Placed these in the refrigerator. - Prepared stocks of bacteria for future use. 6.5 Bacteria Cultures 3/20/13 3/22/13 3/25/13 5/24/13 - Ran a CsCl gradient to further purify phage from 5/26/13 - Began the PEG purification. 5.26 PEG Purification