Team:BYU Provo/Notebook/Phage Purification/Springexp/Period1/Dailylog

From 2013.igem.org

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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: June 2 - June 8 Daily Log'''</font>
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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: May 1 - May 12 Daily Log'''</font>
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<font size="4"> '''6/3/13''' </font>
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<font size="4"> '''5/1/13''' </font>
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- Ran the CsCl purification step.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]]
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- Next class we will attempt extracting the phage from the gradient.
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Today we created a detailed tentative plan to follow for the rest of the semester. https://docs.google.com/document/d/1ffqtGWjGOqEuOgNd46yK38lzkzMVdoCgXPcxMcWfCcc/edit?usp=sharing We went through the protein purification procedure to figure out the times that each step would take. https://docs.google.com/document/d/1W56-4bIIKsWBDtxsY2VgwV1q5BTo2nIXmrOLuqA0U9A/edit?usp=sharing
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Revision as of 23:46, 7 June 2013


Phage Purification May - June Notebook: May 1 - May 12 Daily Log



Overview
March-April
May-June
July-August
September-October


5/1/13

Today we created a detailed tentative plan to follow for the rest of the semester. https://docs.google.com/document/d/1ffqtGWjGOqEuOgNd46yK38lzkzMVdoCgXPcxMcWfCcc/edit?usp=sharing We went through the protein purification procedure to figure out the times that each step would take. https://docs.google.com/document/d/1W56-4bIIKsWBDtxsY2VgwV1q5BTo2nIXmrOLuqA0U9A/edit?usp=sharing

6/5/13

- Extracted the phage from 6.3 CsCl Gradient Phage Purification using a syringe and a needle.

Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
Placed these in the refrigerator.

- Prepared stocks of bacteria for future use.

6.5 Bacteria Cultures


3/20/13



3/22/13


3/25/13