Team:UNITN-Trento/Parts

From 2013.igem.org

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<span class="title">Bacillus</span>
<span class="title">Bacillus</span>
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<table>
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<td rowspan="2" class="grade">☺ ☺ ☺</td>
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<td class="code">BBa_K1065201</td>
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<td class="name">pXyl+GFP</td>
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</tr>
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<tr>
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<td colspan="2" class="descr">
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An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.
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</table>
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<div class="part-box">
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<table>
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<tr>
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<td rowspan="2" class="grade">☺ ☺ ☺</td>
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<td class="code">BBa_K1065106</td>
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<td class="name">BSMT1, PchBA and SAM synthetase</td>
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</tr>
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<tr>
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<td colspan="2" class="descr">
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A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).
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<div class="part-box">
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<table>
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<tr>
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<td rowspan="2" class="grade">☺ ☺ ☺</td>
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<td class="code">BBa_K1065204</td>
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<td class="name">pXyl+EFE</td>
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</tr>
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<tr>
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<td colspan="2" class="descr">
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EFE in pXyl vector, specific for B. subtilis.
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</table>
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Revision as of 14:39, 19 September 2013

Ethylene
☺ ☺ ☺ BBa_K1065000 EFE
Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate.
☺ ☺ ☺ BBa_K1065002 EFE with double-terminator
EFE followed by the double terminator BBa_B0015.
☺ ☺ ☺ BBa_K1065001 EFE arabinose-regulated
This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.
Methyl salicylate
☺ ☺ ☺ BBa_K1065100 BSMT1
This part encodes the BSMT1, it is the enzyme that methylates salycilic acid to obtain methyl salycilate. It’s the original part from the Eau de coli MIT iGEM 2006 project, resubmitted by us in pSB1C3.
☺ ☺ ☺ BBa_K1065103 PchBA
PchA ,the enzyme catalyzing salicylate biosynthesis isochorismate, and PchB that is the isochorismate-pyruvate lyase for that form the precursor for the salycilic acid.
☺ ☺ ☺ BBa_K1065104 SAM synthetase
This part encodes the SAM (S-AdoMet) synthetase gene, (which we friendly called "uncle SAM") . It is a methyl donor required to produce MeSA and other methylated molecules.
☺ ☺ ☺ BBa_K1065101 BSMT1 arabinose-regulated
A methyltransferase (BSMT1) regulated by the araCpBAD inducible promoter.
☺ ☺ ☺ Bba_K1065105 SAM synthetase IPTG-regulated
This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.
☺ ☺ ☺ BBa_K1065102 PchBA IPTG-regulated and BSMT1 arabinose-regulated
This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.
☺ ☺ ☺ BBa_K1065106 BSMT1, PchBA and SAM synthetase
An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.
Blue light
Bacillus
☺ ☺ ☺ BBa_K1065201 pXyl+GFP
An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.
☺ ☺ ☺ BBa_K1065106 BSMT1, PchBA and SAM synthetase
A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).
☺ ☺ ☺ BBa_K1065204 pXyl+EFE
EFE in pXyl vector, specific for B. subtilis.
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