Team:UNITN-Trento/Parts

From 2013.igem.org

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Revision as of 07:56, 21 September 2013

Ethylene

	BBa_K1065000	EFE
	Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate.

	BBa_K1065002	EFE with double-terminator
	EFE followed by the double terminator BBa_B0015.

	BBa_K1065001	EFE arabinose-regulated
	This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

Methyl salicylate

	BBa_K1065100	BSMT1
	This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the 'Eau de coli' MIT iGEM 2006 project, re-submitted (and resurrected) by us in pSB1C3.

	BBa_K1065103	PchBA
	PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid.

	BBa_K1065104	SAM synthetase
	This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules.

	BBa_K1065101	BSMT1 arabinose-regulated
	A methyltransferase (BSMT1) regulated by the araCpBAD promoter.

	BBa_K1065105	SAM synthetase under pLac promoter
	This is SAM synthetase (uncle SAM) regulated by the constitutive Lac promoter.

	BBa_K1065102	PchBA under pLac and BSMT1 arabinose-regulated
	The wintergreen device: PchBA under a mutant Lac promoter (BBa_K1065107) followed by BSMT1 under the araCpBAD promoter.

	BBa_K1065106	BSMT1, PchBA and SAM synthetase
	An improved version of the wintergreen device: BBa_K1065102 with SAM synthetase to boost production.

Blue light

	Bba_K1065301	amilCP IPTG-regulated
	We attached pLac promoter to the amilCP gene. This part does not work. In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). No RBS, no party!

	BBa_K1065306	J23100+Yf1+FixJ
	Yf1 and FixJ under the Anderson promoter (BBa_J23100).

	BBa_K1065303	Yf1+FixJ+pFixK2
	The starting point to build a blue-light sensing circuit: the photosensor (Yf1)and the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006.

	Bba_K1065300	BBa_K952003 with Yf1 and FixJ IPTG-regulated.
	pLac+Yf1-FixJ-pFixK2-amil GFP without RBS and with pLac upstream.

	BBa_K1065310	Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP
	This is a complete blue-light sensing circuit, inverted with cI. Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.

	BBa_K1065302	BBa_K1065300 improved
	The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now it works!!! Just to remember: pLac+Yf1+FixJ+pFixK2+amilGFP.

	BBa_K1065305	BBa_K952003 improved
	The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! Just to remember: Yf1+FixJ+pFixK2+amilGFP.

	BBa_K1065204	pXyl+EFE
	EFE in pXyl vector, specific for B. subtilis.

	BBa_K1065309	BBa_K1065302+BBa_K1065002
	Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE.

Bacillus subtilis

	BBa_K1065201	pXyl+GFP
	An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.

	BBa_K1065106	BSMT1, PchBA and SAM synthetase
	A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).

	BBa_K1065204	pXyl+EFE
	EFE in pXyl vector, specific for B. subtilis.

	BBa_K1065311	Complete blue-light sensing device with inverter, amilCP and EFE
	Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE.

[http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Parts&action=edit Edit this page] | Main Page

@@ Line 185: / Line 185: @@
                      <tr>
                          <td colspan="2" class="descr">
-                             This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.
+                             The wintergreen device: PchBA under a mutant Lac promoter (BBa_K1065107) followed by BSMT1 under the araCpBAD promoter.
                          </td>
                      </tr>
@@ Line 204: / Line 204: @@
                      <tr>
                          <td colspan="2" class="descr">
-                             An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.
+                             An improved version of the wintergreen device: BBa_K1065102 with SAM synthetase to boost production.
                          </td>
                      </tr>
@@ Line 221: / Line 221: @@
                      <tr>
                          <td rowspan="2" class="grade">
-                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                              <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                          </td>
@@ Line 230: / Line 228: @@
                      <tr>
                          <td colspan="2" class="descr">
-                             We attached pLac promoter to amilCP gene. This part does not work (sadly we saw no blue color). In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). <i>No RBS, no party!</i>
+                             We attached pLac promoter to the amilCP gene. This part does not work. In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). <i>No RBS, no party!</i>
                          </td>
                      </tr>
@@ Line 244: / Line 242: @@
                              <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                          </td>
-                         <td class="code">Bba_K1065300</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065306">BBa_K1065306</a></td>
-                         <td class="name">BBa_K952003 with Yf1 and FixJ IPTG-regulated.</td>
+                         <td class="name">J23100+Yf1+FixJ</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             pLac+Yf1-FixJ-pFixK2-amil GFP without RBS and with pLac upstream.
+                             Yf1 and FixJ under the Anderson promoter (BBa_J23100).
                          </td>
                      </tr>
@@ Line 259: / Line 257: @@
                      <tr>
                          <td rowspan="2" class="grade">
-                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                              <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                              <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
@@ Line 268: / Line 265: @@
                      <tr>
                          <td colspan="2" class="descr">
-                             The starting point to build a blue-light sensing circuit: the photosensor, the response regulator with its promoter. This part was build merging BBa_K592016 and BBa_K592006.
+                             The starting point to build a blue-light sensing circuit: the photosensor (Yf1)and  the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006.
                          </td>
                      </tr>
@@ Line 278: / Line 275: @@
                      <tr>
                          <td rowspan="2" class="grade">
-                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
-                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                              <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                          </td>
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065310">BBa_K1065310</a></td>
+                         <td class="code">Bba_K1065300</td>
-                         <td class="name">Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP</td>
+                         <td class="name">BBa_K952003 with Yf1 and FixJ IPTG-regulated.</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             This is a complete blue-light sensing circuit, inverted with cI. Yf1 and FixJ are under the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.
+                             pLac+Yf1-FixJ-pFixK2-amil GFP without RBS and with pLac upstream.
                          </td>
                      </tr>
@@ Line 301: / Line 296: @@
                              <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                          </td>
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065302">BBa_K1065302</a></td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065310">BBa_K1065310</a></td>
-                         <td class="name">BBa_K1065300 improved</td>
+                         <td class="name">Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now this works!!! It is: pLac+Yf1+FixJ+pFixK2+amilGFP.
+                             This is a complete blue-light sensing circuit, inverted with cI. Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.
                          </td>
                      </tr>
@@ Line 316: / Line 311: @@
                      <tr>
                          <td rowspan="2" class="grade">
-                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                              <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                              <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                          </td>
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065305">BBa_K1065305</a></td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065302">BBa_K1065302</a></td>
-                         <td class="name">BBa_K952003 improved</td>
+                         <td class="name">BBa_K1065300 improved</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! It is: Yf1+FixJ+pFixK2+amilGFP.
+                             The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now it works!!! Just to remember: pLac+Yf1+FixJ+pFixK2+amilGFP.
                          </td>
                      </tr>
@@ Line 339: / Line 333: @@
                              <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                          </td>
-                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065306">BBa_K1065306</a></td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065305">BBa_K1065305</a></td>
-                         <td class="name">J23100+Yf1+FixJ</td>
+                         <td class="name">BBa_K952003 improved</td>
                      </tr>
                      <tr>
                          <td colspan="2" class="descr">
-                             Yf1 and FixJ under the Anderson promoter (BBa_J23100).
+                             The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! Just to remember: Yf1+FixJ+pFixK2+amilGFP.
                          </td>
                      </tr>