Team:UNITN-Trento/Parts

From 2013.igem.org

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                         </td>
                         </td>
                         <td class="code">Bba_K1065301</td>
                         <td class="code">Bba_K1065301</td>
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                         <td class="name">amilCP IPTG-regulated</td>
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                         <td class="name">amilCP under pLac promoter</td>
                     </tr>
                     </tr>
                     <tr>
                     <tr>
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                     <tr>
                     <tr>
                         <td rowspan="2" class="grade">
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                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
 
                             <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                             <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                             <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                             <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
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                     <tr>
                     <tr>
                         <td colspan="2" class="descr">
                         <td colspan="2" class="descr">
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                             Yf1 and FixJ under the Anderson promoter (BBa_J23100).
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                             Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100).
                         </td>
                         </td>
                     </tr>
                     </tr>
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                     <tr>
                     <tr>
                         <td colspan="2" class="descr">
                         <td colspan="2" class="descr">
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                             The starting point to build a blue-light sensing circuit: the photosensor (Yf1)and  the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006.
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                             The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and  the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006.
                         </td>
                         </td>
                     </tr>
                     </tr>
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                         </td>
                         </td>
                         <td class="code">Bba_K1065300</td>
                         <td class="code">Bba_K1065300</td>
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                         <td class="name">BBa_K952003 with Yf1 and FixJ IPTG-regulated.</td>
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                         <td class="name">BBa_K952003 with Yf1 and FixJ under pLac promoter.</td>
                     </tr>
                     </tr>
                     <tr>
                     <tr>
                         <td colspan="2" class="descr">
                         <td colspan="2" class="descr">
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                             pLac+Yf1-FixJ-pFixK2-amil GFP without RBS and with pLac upstream.
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                             pLac+Yf1-FixJ-pFixK2-amil GFP constructed adding the pLac promoter upstream of BBa_K952003, which lacks the RBS before amil GFP.
                         </td>
                         </td>
                     </tr>
                     </tr>
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                         <td rowspan="2" class="grade">
                         <td rowspan="2" class="grade">
 +
                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                             <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                             <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
                             <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
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                         <td colspan="2" class="descr">
                         <td colspan="2" class="descr">
                             The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! Just to remember: Yf1+FixJ+pFixK2+amilGFP.
                             The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! Just to remember: Yf1+FixJ+pFixK2+amilGFP.
-
                        </td>
 
-
                    </tr>
 
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                </table>
 
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            </div>
 
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            <div class="part-box">
 
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                <table>
 
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                    <tr>
 
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                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
 
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                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
 
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                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
 
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                        </td>
 
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                        <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065204">BBa_K1065204</a></td>
 
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                        <td class="name">pXyl+EFE</td>
 
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                    </tr>
 
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                    <tr>
 
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                        <td colspan="2" class="descr">
 
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                            EFE in pXyl vector, specific for B. subtilis.
 
                         </td>
                         </td>
                     </tr>
                     </tr>
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             </div>
             </div>
             <span class="title"><a id="bacillus"><i>Bacillus subtilis</i></a></span>
             <span class="title"><a id="bacillus"><i>Bacillus subtilis</i></a></span>
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 +
            <div class="part-box">
 +
                <table>
 +
                    <tr>
 +
                        <td rowspan="2" class="grade">
 +
                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
 +
                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
 +
                            <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />
 +
                        </td>
 +
                        <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065204">BBa_K1065204</a></td>
 +
                        <td class="name">pXyl+EFE</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td colspan="2" class="descr">
 +
                            EFE in pXyl vector, specific for B. subtilis.
 +
                        </td>
 +
                    </tr>
 +
                </table>
 +
            </div>
             <div class="part-box">
             <div class="part-box">

Revision as of 08:16, 21 September 2013

Ethylene Methyl salicylate Blue light B. subtilis
Ethylene
BBa_K1065000 EFE
Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate.
BBa_K1065002 EFE with double-terminator
EFE followed by the double terminator BBa_B0015.
BBa_K1065001 EFE arabinose-regulated
This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.
Methyl salicylate
BBa_K1065100 BSMT1
This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the 'Eau de coli' MIT iGEM 2006 project, re-submitted (and resurrected) by us in pSB1C3.
BBa_K1065103 PchBA
PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid.
BBa_K1065104 SAM synthetase
This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules.
BBa_K1065101 BSMT1 arabinose-regulated
A methyltransferase (BSMT1) regulated by the araCpBAD promoter.
BBa_K1065105 SAM synthetase under pLac promoter
This is SAM synthetase (uncle SAM) regulated by the constitutive Lac promoter.
BBa_K1065102 PchBA under pLac and BSMT1 arabinose-regulated
The wintergreen device: PchBA under a mutant Lac promoter (BBa_K1065107) followed by BSMT1 under the araCpBAD promoter.
BBa_K1065106 BSMT1, PchBA and SAM synthetase
An improved version of the wintergreen device: BBa_K1065102 with SAM synthetase to boost production.
Blue light
Bba_K1065301 amilCP under pLac promoter
We attached pLac promoter to the amilCP gene. This part does not work. In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). No RBS, no party!
BBa_K1065306 J23100+Yf1+FixJ
Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100).
BBa_K1065303 Yf1+FixJ+pFixK2
The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006.
Bba_K1065300 BBa_K952003 with Yf1 and FixJ under pLac promoter.
pLac+Yf1-FixJ-pFixK2-amil GFP constructed adding the pLac promoter upstream of BBa_K952003, which lacks the RBS before amil GFP.
BBa_K1065310 Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP
This is a complete blue-light sensing circuit, inverted with cI. Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.
BBa_K1065302 BBa_K1065300 improved
The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now it works!!! Just to remember: pLac+Yf1+FixJ+pFixK2+amilGFP.
BBa_K1065305 BBa_K952003 improved
The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! Just to remember: Yf1+FixJ+pFixK2+amilGFP.
      BBa_K1065309 BBa_K1065302+BBa_K1065002
Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE.
Bacillus subtilis
BBa_K1065204 pXyl+EFE
EFE in pXyl vector, specific for B. subtilis.
      BBa_K1065201 pXyl+GFP
An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.
      BBa_K1065106 BSMT1, PchBA and SAM synthetase
A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).
      BBa_K1065204 pXyl+EFE
EFE in pXyl vector, specific for B. subtilis.
      BBa_K1065311 Complete blue-light sensing device with inverter, amilCP and EFE
Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE.
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