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Team:UNITN-Trento/Parts
From 2013.igem.org
(Difference between revisions)
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<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE. | Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE. | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | </div> | ||
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| + | <div class="part-box"> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> </td> | ||
| + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065311">BBa_K1065311</a></td> | ||
| + | <td class="name">Complete blue-light sensing device with inverter, amilCP and EFE</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td colspan="2" class="descr"> | ||
| + | Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE. | ||
</td> | </td> | ||
</tr> | </tr> | ||
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<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
EFE in pXyl vector, specific for B. subtilis. | EFE in pXyl vector, specific for B. subtilis. | ||
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</td> | </td> | ||
</tr> | </tr> | ||
Revision as of 08:16, 21 September 2013
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BBa_K1065000 | EFE |
| Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate. | ||
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BBa_K1065002 | EFE with double-terminator |
| EFE followed by the double terminator BBa_B0015. | ||
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BBa_K1065001 | EFE arabinose-regulated |
| This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter. | ||
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BBa_K1065100 | BSMT1 |
| This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the 'Eau de coli' MIT iGEM 2006 project, re-submitted (and resurrected) by us in pSB1C3. | ||
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BBa_K1065103 | PchBA |
| PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid. | ||
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BBa_K1065104 | SAM synthetase |
| This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules. | ||
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BBa_K1065101 | BSMT1 arabinose-regulated |
| A methyltransferase (BSMT1) regulated by the araCpBAD promoter. | ||
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BBa_K1065105 | SAM synthetase under pLac promoter |
| This is SAM synthetase (uncle SAM) regulated by the constitutive Lac promoter. | ||
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BBa_K1065102 | PchBA under pLac and BSMT1 arabinose-regulated |
| The wintergreen device: PchBA under a mutant Lac promoter (BBa_K1065107) followed by BSMT1 under the araCpBAD promoter. | ||
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BBa_K1065106 | BSMT1, PchBA and SAM synthetase |
| An improved version of the wintergreen device: BBa_K1065102 with SAM synthetase to boost production. | ||
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Bba_K1065301 | amilCP under pLac promoter |
| We attached pLac promoter to the amilCP gene. This part does not work. In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). No RBS, no party! | ||
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BBa_K1065306 | J23100+Yf1+FixJ |
| Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100). | ||
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BBa_K1065303 | Yf1+FixJ+pFixK2 |
| The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006. | ||
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Bba_K1065300 | BBa_K952003 with Yf1 and FixJ under pLac promoter. |
| pLac+Yf1-FixJ-pFixK2-amil GFP constructed adding the pLac promoter upstream of BBa_K952003, which lacks the RBS before amil GFP. | ||
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BBa_K1065310 | Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP |
| This is a complete blue-light sensing circuit, inverted with cI. Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced. | ||
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BBa_K1065302 | BBa_K1065300 improved |
| The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now it works!!! Just to remember: pLac+Yf1+FixJ+pFixK2+amilGFP. | ||
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BBa_K1065305 | BBa_K952003 improved |
| The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! Just to remember: Yf1+FixJ+pFixK2+amilGFP. | ||
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BBa_K1065309 | BBa_K1065302+BBa_K1065002 |
| Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE. | ||
| |
BBa_K1065311 | Complete blue-light sensing device with inverter, amilCP and EFE |
| Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE. | ||
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BBa_K1065204 | pXyl+EFE |
| EFE in pXyl vector, specific for B. subtilis. | ||
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BBa_K1065201 | pXyl+GFP |
| An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production. | ||
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BBa_K1065106 | BSMT1, PchBA and SAM synthetase |
| A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840). | ||
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BBa_K1065204 | pXyl+EFE |
| EFE in pXyl vector, specific for B. subtilis. | ||
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