Team:UNITN-Trento/Project/Datapage

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Revision as of 14:12, 23 September 2013

We envision this genetic circuit in wich production of ethylene and MeSA is regulated by light. In a dark state, the sensor protein YF-1 auto-phosphorilates itself, dimerize, and transfer the phosphate to the response regulator element FixJ. FixJ is so activated and is able to bind pFixK2 promoter, activating transcription of cI (inhibitor protein) that ultimately blocks EFE production and activating transcriptional Bmst1, wich regulates MeSA synthesis. In a blue-light state the sensor protein YF-1 does not phosphorilate itself, so pFixK2 promoters is in the off state: ethylene is produced thus promoting ripening while MeSA production is blocked.

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-			This image shows our ideal and final circuit. In a dark state, the sensor protein YF-1 auto-phosphorilates itself and then pass the phosphate to the response regulator element FixJ. FixJ is so activated and is able to bind pFixK2 promoters and activate transcription of cI (inhibitor protein) that blocks EFE transcription and Bmst1, the gene necessary for the last step in MeSA synthesis. In a blue-light state the sensor protein YF-1 does not phosphorilate itself, so pFixK2 promoters are not activated: ethylene is produced while MeSA is not.
+			We envision this genetic circuit in wich production of ethylene and MeSA is regulated by light. In a dark state, the sensor protein YF-1 auto-phosphorilates itself, dimerize, and transfer the phosphate to the response regulator element FixJ. FixJ is so activated and is able to bind pFixK2 promoter, activating transcription of cI (inhibitor protein) that ultimately blocks EFE production and activating transcriptional Bmst1, wich regulates MeSA synthesis. In a blue-light state the sensor protein YF-1 does not phosphorilate itself, so pFixK2 promoters is in the off state: ethylene is produced thus promoting ripening while MeSA production is blocked.
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