Team:BYU Provo/Notebook/Phage Purification/Springexp/Period2/Dailylog

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<font size="4"> '''5/29/13''' </font>
 
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- Finished the PEG purification.
 
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.26_PEG_Purification|5.26 PEG Purification]]
 
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<font size="4"> '''5/31/13''' </font>
 
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- Worked on setting up our notebook online.
 
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<font size="4"> '''6/3/13''' </font>
 
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- Ran the CsCl purification step.
 
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]]
 
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- Next class we will attempt extracting the phage from the gradient.
 
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<font size="4"> '''6/5/13''' </font>
 
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- Extracted the phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] using a syringe and a needle. 
 
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: Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
 
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: Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
 
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: Placed these in the refrigerator.
 
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- Prepared stocks of bacteria for future use.
 
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.5 Bacteria Cultures|6.5 Bacteria Cultures]]
 
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<font size="4"> '''3/20/13''' </font>
 
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<font size="4"> '''3/22/13''' </font>
 
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Revision as of 22:04, 17 June 2013


Phage Purification May - June Notebook: May 13 - May 26 Daily Log

Overview
March-April
May-June
July-August
September-October


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5/22/13

- Repeated T4 titer as the last titer showed contamination.

5.22 T4 Titer


5/24/13

- Ran a CsCl gradient to further purify phage from


5/26/13

- Began the PEG purification.

5.26 PEG Purification




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