Team:HIT-Harbin
From 2013.igem.org
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- | <h4 class="hashed"><span> | + | <h4 class="hashed"><span>The circuit</span></h4> |
- | + | <p>The following picture is the whole design of our circuit.</p> | |
- | + | <div align="center"> | |
- | + | <img src="https://static.igem.org/mediawiki/2013/7/75/HIT-Harbin_Project_Andgate.png" /> | |
- | + | <p>Figure 1 : A Schematic of the Biological Andgate</p> | |
- | + | </div> | |
- | + | <p>As showed in Fig. 1, a protein complex HrpRS is formed following the combination of HrpS and HrpR. HrpRS then binds to the promoter hrpL, triggering transcription of the downstream gene. Two inducible promoters--the isopropylthiogalactoside (IPTG)-inducible Plac and the arabinose-inducible PBAD – are selected as the AND gate inputs. A model based on protein and protein-promoter interactions for the hrp gene regulation is described below:</p> | |
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Revision as of 19:08, 24 September 2013
Home
The circuit
The following picture is the whole design of our circuit.
![](https://static.igem.org/mediawiki/2013/7/75/HIT-Harbin_Project_Andgate.png)
Figure 1 : A Schematic of the Biological Andgate
As showed in Fig. 1, a protein complex HrpRS is formed following the combination of HrpS and HrpR. HrpRS then binds to the promoter hrpL, triggering transcription of the downstream gene. Two inducible promoters--the isopropylthiogalactoside (IPTG)-inducible Plac and the arabinose-inducible PBAD – are selected as the AND gate inputs. A model based on protein and protein-promoter interactions for the hrp gene regulation is described below:
Learn More
Click here and get more details about our project.