Team:USTC CHINA/Notebook/Protocols

From 2013.igem.org

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<h1>Transformation</h1>
<h1>Transformation</h1>
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<h2.The transformation of E.coli</h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/The transformation of E.coli">The transformation of E.coli</a></h2>
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<h2>The transformation of Bacillus subtilis</h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/The transformation of Bacillus subtilis">The transformation of Bacillus subtilis</a></h2>
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<h1>Overlap PCR</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Overlap PCR">Overlap PCR</a></h1>
<h1>Extracting plasmids</h1>
<h1>Extracting plasmids</h1>
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<h2>Extracting plasmids from gram-positive bacterium</h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Extracting plasmids from gram-positive bacterium">Extracting plasmids from gram-positive bacterium</a></h2>
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<h2>Extracting plasmids from gram-negative bacterium<h2></div>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Extracting plasmids from gram-negative bacterium">Extracting plasmids from gram-negative bacterium</a><h2></div>
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Revision as of 13:00, 25 September 2013

Expression of proteins

Expression of proteins in Bacillus subtilis

Expression of proteins in Escherichia coli

Concentrating proteins

Transdermal experiments Immunity&Tests

ELISA

Sample analysis

Medium

LB media

10*S base

HS media

LS media

2*YT media

Measurement

Constitutive promoter measurements

plac promoter response to IPTG or Glucose