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Team:USTC CHINA/Notebook/Protocols/Expression of proteins in Bacillus subtilis
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<h1>Expression of proteins in Bacillus subtilis</h1> | <h1>Expression of proteins in Bacillus subtilis</h1> | ||
| - | <p>Induction with IPTG | + | <p><span>Induction with IPTG</span></br> |
| - | - grow appropriate B. subtilis strain overnight in fresh 2xYT medium | + | - grow appropriate B. subtilis strain overnight in fresh 2xYT medium</br> |
| - | - inoculate into fresh 2xYT medium to an OD600 of 0.15 | + | - inoculate into fresh 2xYT medium to an OD600 of 0.15</br> |
| - | - when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM IPTG | + | - when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM IPTG(CB-0487-1GAM) to one portion (t = 0)</br> |
| - | (CB-0487-1GAM) to one portion (t = 0) | + | - collect samples at different time points for analysis (t = 1,…)</br> |
| - | - collect samples at different time points for analysis (t = 1,…) | + | |
</p></div> | </p></div> | ||
</div> | </div> | ||
Revision as of 15:00, 25 September 2013
