Team:USTC CHINA/Notebook/Protocols/Constitutive promoter measurements

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<h1>Constitutive promoter measurements</h1>
<h1>Constitutive promoter measurements</h1>
<p>1. Streak a LB plate of the strain.</br>
<p>1. Streak a LB plate of the strain.</br>
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2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic with single colony <ul>aaa</ul>from the plate.</br>
+
2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic with single colony <li></li>from the plate.</br>
3. Cultures were grown in test tubes for 16hrs at 37℃ with shaking at 200rpm.</br>
3. Cultures were grown in test tubes for 16hrs at 37℃ with shaking at 200rpm.</br>
4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.</br>
4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.</br>

Revision as of 16:18, 25 September 2013

Constitutive promoter measurements

1. Streak a LB plate of the strain.
2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic with single colony

  • from the plate.
    3. Cultures were grown in test tubes for 16hrs at 37℃ with shaking at 200rpm.
    4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.
    5. Measure the fluorescence (performed with SpectraMax M5 Multi-Mode Microplate Reader，add 200ul liquid in each well) and absorbance (HITACHI UV-VIS spectrophotometer U-2810 ,200ul quartz cell,path length 10mm,600nm,1.5 nm slit width) every 30 minutes in the next 4hrs.

    notebook

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