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The beast and its Achilles heel:
A novel target to fight multi-resistant pathogenic bacteria
Two members of our team went to the iGEM Team Groningen to perform microarray analyses. With this approach we tried to get a closer look into cyclic-di-AMP influenced gene regulation.
Two members of our team with the Groningen iGEM Team.
We were interested in the expression levels of ydaO under different conditions, which was our indicator for the “riboswitch” appearance. Of course, we also looked for other genes which could be of interest for our project.
Here Sam is preparing the needed RNA for subsequent microarray analyses.
Besides the lab work, we were also interested what Groningen has to offer during the night. So the Groningen team took our two members to a night out through the nightlife of Groningen.
In some Groningen bar.
In return for helping us with the microarray analyses, we as Team Göttingen tried to help out Groningen in attracting more sponsors. Therefore we showed them how we got in very successful contact with our sponsors.
We sent two soil samples tot he Norwich team. One from the area around our lab and the other from the Wadden Sea.
The department of Prof. Volkhard Kaever at the MHH helped us with determining the concentration of cyclic di-AMP by a liquid chromatography-coupled tandem mass spectrometry method.
The department of Prof. Ralf Ficner helped us with crystallization assays and subsequent improvement of the crystallization conditions. Additionally, the department used the defraction pattern to design the enzymes structure.
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