Team:Hong Kong HKUST/results

From 2013.igem.org

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<div id="slide"><center><h3 class="title">Result</h3></center>
<div id="slide"><center><h3 class="title">Result</h3></center>
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<p id="yo">While low-fat diet and regular exercise are popular approaches to fight with obesity, one easy alternative is simply to increase energy metabolism. In a synthetic biology approach, we are working to create an artificial futile cycle in mammalian cell by introducing glyoxylate enzymes native to bacteria. Past research has shown that mice expressing enzymes constituting an active glyoxylate shunt are shown to be resistant to diet-induced obesity. Our team plans to introduce an inducible system that allows us to couple the sensing of circulating fatty acid concentrations with an inducible circuit of glyoxylate shunt. Our inducible system is intended to prevent the risk of fatty acid deficiency, while facilitating greater fatty acid uptake at higher fatty acid circulating concentrations. Such a system should increase the feasibility of a glyoxylate cycle engineered to function in vivo. </p>
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<p id="yo">Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:
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  <li>Successfully construct the two parts</a> for mitochondria leader sequence, MLS with RFC 10 and RFC 25, (BBa_K1119000, BBa_K1119001).</li>
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  <li>Successfully construct the </a>part of   aceA and aceB for in glyoxylate shunt (BBa_K1119002, BBa_K1119003). </li>
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  <li>Successfully construct the part of a constitutive regulatory promoter, Human Elongation Factor 1-alpha promoter (BBa_K1119004).</li>
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  <li>Successfully construct the part for a constitutive CMV promoter (BBa_K1119006).</li>
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  <li>Successfully characterized the regulatory promoter <em>pCMV</em> in HEK293FT cells (BBa_K1119008).</li>
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  <li>Successfully characterized the mitochondria leader sequence MLS in HEK293FT cells (BBa_K1119009).</li>
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  <li>Successfully tested the HepG2 cell viability in different sodium palmitate concentrations. </li>
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Revision as of 11:30, 26 September 2013




Result

Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:

  • Successfully construct the two parts for mitochondria leader sequence, MLS with RFC 10 and RFC 25, (BBa_K1119000, BBa_K1119001).
  • Successfully construct the part of aceA and aceB for in glyoxylate shunt (BBa_K1119002, BBa_K1119003).
  • Successfully construct the part of a constitutive regulatory promoter, Human Elongation Factor 1-alpha promoter (BBa_K1119004).
  • Successfully construct the part for a constitutive CMV promoter (BBa_K1119006).
  • Successfully characterized the regulatory promoter pCMV in HEK293FT cells (BBa_K1119008).
  • Successfully characterized the mitochondria leader sequence MLS in HEK293FT cells (BBa_K1119009).
  • Successfully tested the HepG2 cell viability in different sodium palmitate concentrations.



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