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Team:Hong Kong HKUST/results
From 2013.igem.org
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<p id="yo">Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below: | <p id="yo">Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below: | ||
<ul> | <ul> | ||
| - | <li>Successfully constructed the two parts</a> for mitochondria leader sequence, MLS with RFC 10 and RFC 25, (BBa_K1119000, BBa_K1119001).</li> | + | <li>Successfully constructed the two parts</a> for mitochondria leader sequence, MLS with RFC 10 and RFC 25, (<a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a>).</li> |
| - | <li>Successfully constructed the </a>part of aceA and aceB for in glyoxylate shunt (BBa_K1119002, BBa_K1119003). </li> | + | <li>Successfully constructed the </a>part of aceA and aceB for in glyoxylate shunt (<a href="http://parts.igem.org/Part:BBa_K1119002">BBa_K1119002</a>, <a href="http://parts.igem.org/Part:BBa_K1119003">BBa_K1119003</a>). </li> |
| - | <li>Successfully constructed the part of a constitutive regulatory promoter, Human Elongation Factor 1-alpha promoter (BBa_K1119004).</li> | + | <li>Successfully constructed the part of a constitutive regulatory promoter, Human Elongation Factor 1-alpha promoter (<a href="http://parts.igem.org/Part:BBa_K1119004">BBa_K1119004</a>).</li> |
| - | <li>Successfully construct the part for a constitutive CMV promoter (BBa_K1119006).</li> | + | <li>Successfully construct the part for a constitutive CMV promoter (<a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a>).</li> |
| - | <li>Successfully characterized the regulatory promoter <em>pCMV</em> in HEK293FT cells (BBa_K1119008).</li> | + | <li>Successfully characterized the regulatory promoter <em>pCMV</em> in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a>).</li> |
| - | <li>Successfully characterized the mitochondria leader sequence MLS in HEK293FT cells (BBa_K1119009).</li> | + | <li>Successfully characterized the mitochondria leader sequence MLS in HEK293FT cells (<a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a>).</li> |
<li>Successfully tested the HepG2 cell viability in different sodium palmitate concentrations. </li> | <li>Successfully tested the HepG2 cell viability in different sodium palmitate concentrations. </li> | ||
</ul></p> | </ul></p> | ||
Revision as of 13:00, 26 September 2013
Result
Our project was first proposed in mid-March and after a series of improvement and elaboration, we started our wet lab work from mid-June to late-September. After 3 months of memorable diligent work, we are proud to claim that we have achieved the results below:
- Successfully constructed the two parts for mitochondria leader sequence, MLS with RFC 10 and RFC 25, (BBa_K1119000, BBa_K1119001).
- Successfully constructed the part of aceA and aceB for in glyoxylate shunt (BBa_K1119002, BBa_K1119003).
- Successfully constructed the part of a constitutive regulatory promoter, Human Elongation Factor 1-alpha promoter (BBa_K1119004).
- Successfully construct the part for a constitutive CMV promoter (BBa_K1119006).
- Successfully characterized the regulatory promoter pCMV in HEK293FT cells (BBa_K1119008).
- Successfully characterized the mitochondria leader sequence MLS in HEK293FT cells (BBa_K1119009).
- Successfully tested the HepG2 cell viability in different sodium palmitate concentrations.
