Team:HIT-Harbin/Parts
From 2013.igem.org
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<p></p>CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69% | <p></p>CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69% | ||
+ | <h4>reference</h4> | ||
<p></p>[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33 | <p></p>[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33 | ||
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Revision as of 13:35, 26 September 2013
Parts
Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote
CODON OPTIMIZATION FOR HRPS AND HRPR GENE
Figure1: Codon Adaptation of hrpS gene in E.coli before optimization
Figure2: Codon Adaptation of hrpS gene in E.coli after optimization
Figure3: Codon Adaptation of hrpR gene in E.coli before optimization
Figure4: Codon Adaptation of hrpR gene in E.coli after optimization
reference
[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33<groupparts>iGEM013 HIT-Harbin</groupparts>