"
Team:Tokyo Tech/Submitted Parts
From 2013.igem.org
| Line 41: | Line 41: | ||
<h2> | <h2> | ||
<p> | <p> | ||
| - | M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell. This biobrick is extracted from M13mp18 phage vector by PCR. It inclueds 11 ORFs, M13 origin, a packaging sequence and lac promoter. The promoter on the upstream of g2 (gene 2) is altered to lux promoter. A phage particle is formed only when the host cell receives AHL signal (3OC6HSL, C6) because g2p (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle. As a reporter, GFP is inserted on the downstream of the lac promoter. | + | M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. This biobrick is extracted from M13mp18 phage vector by PCR. It inclueds 11 ORFs, M13 origin, a packaging sequence and lac promoter. The promoter on the upstream of g2 (gene 2) is altered to lux promoter. A phage particle is formed only when the host cell receives AHL signal (3OC6HSL, C6) because g2p (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle. As a reporter, GFP is inserted on the downstream of the lac promoter. |
</p> | </p> | ||
</h2> | </h2> | ||
| Line 53: | Line 53: | ||
<h2> | <h2> | ||
<p> | <p> | ||
| - | We improved a phosphate sensor part since the existing phosphate sensor part (OUC-China 2012, BBa_K737024) did not have sufficient data. We constructed this part by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part. | + | We improved a phosphate sensor part since the existing phosphate sensor part (OUC-China 2012, BBa_K737024) did not have sufficient data. We constructed this part by amplifying the phoA promoter region of <i>E. coli</i> (MG1655) and ligating it upstream of GFP part. |
Compared to OUC-China’s phosphate sensor part including phoB promoter (Fig. 2), our phosphate sensor part shows clearer result (Fig. 1). | Compared to OUC-China’s phosphate sensor part including phoB promoter (Fig. 2), our phosphate sensor part shows clearer result (Fig. 1). | ||
</p> | </p> | ||
Revision as of 13:36, 26 September 2013
Contents |
Parts submitted to the Registry
For a brief overview of our main results, please have a look at our Main Results page. [Go to “Home”]
Favorite Tokyo_Tech 2013 iGEM Team Parts
| Name | Type | Description | Designer | Length | |
| W | [http://parts.igem.org/Part:BBa_K1139021 BBa_K1139021] | Composite | Plux-M13-Plac-GFP | Naoki Watarai | 7705 |
| W | [http://parts.igem.org/Part:BBa_K1139110 BBa_K1139110] | Composite | Pcon-LasR-Plux/tet-GFP | Naoki Watarai | 1888 |
| W | [http://parts.igem.org/Part:BBa_K1139201 BBa_K1139201] | Measurement | PphoA-GFP-TT | Sara Ogino | 1017 |
Tokyo_Tech 2013 iGEM Team Parts
| Name | Type | Description | Designer | Length | |
| [http://parts.igem.org/Part:BBa_K1139019 BBa_K1139019] | Composite | Promoterless-M13 | Naoki Watarai | 6400 | |
| W | [http://parts.igem.org/Part:BBa_K1139020 BBa_K1139020] | Composite | PlacIQ-M13-Plac-GFP | Naoki Watarai | 7406 |
| [http://parts.igem.org/Part:BBa_K1139150 BBa_K1139150] | Measurement | PRM/lac-GFP-TT | Naoki Watarai | 1032 |
Best new BioBrick part (natural): BBa_K1139021
M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell. This biobrick is extracted from M13mp18 phage vector by PCR. It inclueds 11 ORFs, M13 origin, a packaging sequence and lac promoter. The promoter on the upstream of g2 (gene 2) is altered to lux promoter. A phage particle is formed only when the host cell receives AHL signal (3OC6HSL, C6) because g2p (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle. As a reporter, GFP is inserted on the downstream of the lac promoter.
Best new BioBrick part (engineered): BBa_K1139110
We constructed BBa_K1139110 by combining Pcon-lasR (BBa_K553003) and Plux/tet-GFP (BBa_K934025). This is the first Biobrick part that succeeded in confirming to circumvention of crosstalk. Using this part with plasmid constitutively expression of luxR and tetR, we succeeded in confirming circumvention of crosstalk between AHL signals 3OC6HSL and 3OC12HSL.
Best improved Part: BBa_K1139201
We improved a phosphate sensor part since the existing phosphate sensor part (OUC-China 2012, BBa_K737024) did not have sufficient data. We constructed this part by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part. Compared to OUC-China’s phosphate sensor part including phoB promoter (Fig. 2), our phosphate sensor part shows clearer result (Fig. 1).
