Overview

T-VACCINE——A vaccine that can initiate immune response by penetrating the skin with the aid of transdermal peptide. From now on, injections are simply history! Based on the theory of user-friendly, a special group of engineering bacteria which produce T-VACCINE is used to create a brand-new "band-aid" that can serve as a guardian of our health .

We have found a kind of transdermal peptide TD-1——a magical molecule that not only enhances the permeability of the skin but also draw filamentous bacteriophages into the skin. By combining the gene fragments of antigen, immune adjuvant LTB and Luman-recruiting factor TNLFα with that of the transdermal peptide, our team got the permeable fusion protein. In order to obtain large amount of extracelluar protein, we chose bacillus subtilis WB800N as our expression engineering bacteria. At last, the universality of our experimental method is verified by the adoption of various antigen of existing vaccine, such as HBsAg, PA and AG85B.

Project contains

For in situ transdermal vaccine is real a giant project, we devide it into four lab parts. And we expect to achieve following goals.

Antigen

As the main part of T-vaccine, TD-1 is fused with antigen protein, so it can penetrate the skin and present antigen. We had designed there kinds of vaccine, which are against hepatitis B, TB and anthrax. Our ELISA test and mice test had proved their antigenicity and immunogenicity,thereby proving that T-vaccine can be generally used.