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Team:Yale/Project Export
From 2013.igem.org
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<li><a href='https://2013.igem.org/Team:Yale/Project_Overview'><span>Project Overview</span></a></li> | <li><a href='https://2013.igem.org/Team:Yale/Project_Overview'><span>Project Overview</span></a></li> | ||
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<li><a href='https://2013.igem.org/Team:Yale/Project_MAGE'><span>Apply Mage</span></a></li> | <li><a href='https://2013.igem.org/Team:Yale/Project_MAGE'><span>Apply Mage</span></a></li> | ||
<li class='active'><a href='https://2013.igem.org/Team:Yale/Project_Export'><span>Introduce Export System</span></a></li> | <li class='active'><a href='https://2013.igem.org/Team:Yale/Project_Export'><span>Introduce Export System</span></a></li> | ||
| - | <li class='last'><a href='https://2013.igem.org/Team:Yale/ | + | <li><a href='https://2013.igem.org/Team:Yale/Project_Bioplastic'><span>Make a Bioplastic</span></a></li> |
| + | <li class='last'><a href='https://2013.igem.org/Team:Yale/Project_Collaboration'><span>Make a Bioplastic</span></a></li> | ||
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Revision as of 00:23, 27 September 2013
Introduce type 1 secretion system to export and extract PLA
- We needed a way to export the PLA once it was synthesized by the E. coli
- We found the paper by Linton 2010 where she focused on exporting PHA from engineered E. coli
- Ms. Linton used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag.
- This allowed the cells to export the PLA since the hlyA tag was attached to the granule
- Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells.
