Team:BIOINT Mexico/Lab work

From 2013.igem.org

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'''Experiment 1: Preparation of growth mediums (MRS and LB) (June 5th, 2013)'''
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10 Agar LB plates
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15 Agar MRS plates
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Notes:
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*(1) 18.6g of Agar MRS were weighted to prepare 300ml of medium
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*(2) 7g of Agar LB were weighted to prepare 200ml of medium
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*(1)  5g of A-MRS were diluted in 80ml of distilled water
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*Another 5g and 170ml of distilled water were added
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*Small lumps were formed in the bottom of the Erlenmeyer flask
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*(2) 7g of Agar LB were distilled in 100ml of distilled water
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*Further 100ml of distilled water were added
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*It wasn’t possible to use a control of the sterilization because there was no autoclave tape
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*The autoclave began heating up
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*The autoclave valve was lowered
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*When the temperature of the autoclave reached 120⁰c, the power was cut to the half
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*The vapor cycle ended and the temperature decreased
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*The temperature reached 105⁰c and the valve was opened. The mediums were left inside the autoclave until de laminar flow cabinet was unoccupied
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*The medium in petri dishes were stored in the refrigerator
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*The growth mediums in storage were revised, they do not show signs of contamination
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17 MRS Agar
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9 LB Agar

Revision as of 04:37, 27 September 2013

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Lab work

Experiment 1: Preparation of growth mediums (MRS and LB) (June 5th, 2013)

10 Agar LB plates

15 Agar MRS plates


Notes:

  • (1) 18.6g of Agar MRS were weighted to prepare 300ml of medium
  • (2) 7g of Agar LB were weighted to prepare 200ml of medium
  • (1) 5g of A-MRS were diluted in 80ml of distilled water
  • Another 5g and 170ml of distilled water were added
  • Small lumps were formed in the bottom of the Erlenmeyer flask
  • (2) 7g of Agar LB were distilled in 100ml of distilled water
  • Further 100ml of distilled water were added
  • It wasn’t possible to use a control of the sterilization because there was no autoclave tape
  • The autoclave began heating up
  • The autoclave valve was lowered
  • When the temperature of the autoclave reached 120⁰c, the power was cut to the half
  • The vapor cycle ended and the temperature decreased
  • The temperature reached 105⁰c and the valve was opened. The mediums were left inside the autoclave until de laminar flow cabinet was unoccupied
  • The medium in petri dishes were stored in the refrigerator
  • The growth mediums in storage were revised, they do not show signs of contamination

17 MRS Agar

9 LB Agar

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