Team:Goettingen/Project/Collaborations

From 2013.igem.org

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<h3 style="">Groningen</h3>
<h3 style="">Groningen</h3>
<strong><a href="https://www.facebook.com/Igem2013Groningen" style="font-size:11pt;color:#596678">Their Facebook</a></strong>
<strong><a href="https://www.facebook.com/Igem2013Groningen" style="font-size:11pt;color:#596678">Their Facebook</a></strong>
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<P>Two members of our team went to the iGEM Team Groningen to perform microarray analyses. With this approach we tried novel cyclic-di-AMP-dependent regulatory elements that control gene expression in <i>B. subtilis</i>.</p>
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<P>Two members of our team went to the iGEM Team Groningen to perform microarray analyses. With this approach we tried to identify novel cyclic-di-AMP-dependent regulatory elements that control gene expression in <i>B. subtilis</i>.</p>
<a href="/Team:Goettingen/Team/Gallery#In_Groningen"><img style="text-align:center" src="https://static.igem.org/mediawiki/2013/7/7b/Goe-groningen_1.JPG" width="500" /></a>
<a href="/Team:Goettingen/Team/Gallery#In_Groningen"><img style="text-align:center" src="https://static.igem.org/mediawiki/2013/7/7b/Goe-groningen_1.JPG" width="500" /></a>
<p style="text-align:center">Two members of our team with the Groningen iGEM Team.</p>
<p style="text-align:center">Two members of our team with the Groningen iGEM Team.</p>
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<p>We were interested in the expression levels of <i>ydaO</i> under different conditions, which was our indicator for the “riboswitch” appearance. Of course, we also looked for other genes which could be of interest for our project.</p>
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<p>We found several interesting candidate genes that seemed to be regulated by intracellular c-di-AMP among them <i>ydaO</i>, a gene that has been recently shown to be controlled by a c-di-AMP-dependent “riboswitch”.</p>
<a href="/Team:Goettingen/Team/Gallery#In_Groningen"><img style="text-align:center" src="https://static.igem.org/mediawiki/2013/6/64/Goe-groningen_Sam.jpg" height="500" /></a>
<a href="/Team:Goettingen/Team/Gallery#In_Groningen"><img style="text-align:center" src="https://static.igem.org/mediawiki/2013/6/64/Goe-groningen_Sam.jpg" height="500" /></a>
<p style="text-align:center">This is Sam who prepares the RNA for microarray analyses.</p>
<p style="text-align:center">This is Sam who prepares the RNA for microarray analyses.</p>

Revision as of 08:21, 27 September 2013





The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria


 

Groningen

Their Facebook

Two members of our team went to the iGEM Team Groningen to perform microarray analyses. With this approach we tried to identify novel cyclic-di-AMP-dependent regulatory elements that control gene expression in B. subtilis.

Two members of our team with the Groningen iGEM Team.

We found several interesting candidate genes that seemed to be regulated by intracellular c-di-AMP among them ydaO, a gene that has been recently shown to be controlled by a c-di-AMP-dependent “riboswitch”.

This is Sam who prepares the RNA for microarray analyses.

Besides the lab work, we were also interested what Groningen has to offer during the night. So the Groningen team took our two members to a night out through the nightlife of Groningen.

In some Groningen bar.

In return for helping us with the microarray analyses, we as Team Göttingen tried to help out Groningen in attracting more sponsors. Therefore we showed them how we got in very successful contact with our sponsors.

To see more pictures about us, please visit our Gallery


Norwich

Their Facebook

We sent two soil samples tot he Norwich team. One from the area around our lab and the other from the Wadden Sea.


Purdue

Their Facebook

We participated in the initial surveys.


Hannover Medical School

The department of Prof. Volkhard Kaever at the MHH helped us with determining the concentration of cyclic di-AMP by a liquid chromatography-coupled tandem mass spectrometry method.


Structural Biochemistry Göttingen

The department of Prof. Ralf Ficner helped us with crystallization assays and subsequent improvement of the crystallization conditions. Additionally, the department used the defraction pattern to design the enzymes structure.

 

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