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Team:Uppsala/promoters
From 2013.igem.org
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| + | <h1> A constitutive promoter (CP) library for Escherichia coli and Lactobacillus genus </h1> | ||
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| + | Depending on how much you want to express your gene, you will need promoters with different strengths. Therefor we wanted to provide a selection of promoters that works in both E. coli and Lactobacillus genus. We synthesized seven promoters based on the CP promoter library in the article “The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." by Peter Ruhdal Jensen and Karin Hammer. These promoters are based on the consensus sequence in promoters from Lactococcus lactis. Bellow you can see our promoters and their name in the part registry. | ||
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| + | • CP1 - BBa_K1033219 | ||
| + | • CP8 - BBa_K1033220 | ||
| + | • CP11 - BBa_K1033221 | ||
| + | • CP29 - BBa_K1033222 | ||
| + | • CP30 - BBa_K1033223 | ||
| + | • CP41 - BBa_K1033224 | ||
| + | • CP44 - BBa_K1033225 | ||
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Revision as of 14:43, 27 September 2013
A constitutive promoter (CP) library for Escherichia coli and Lactobacillus genus
Depending on how much you want to express your gene, you will need promoters with different strengths. Therefor we wanted to provide a selection of promoters that works in both E. coli and Lactobacillus genus. We synthesized seven promoters based on the CP promoter library in the article “The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." by Peter Ruhdal Jensen and Karin Hammer. These promoters are based on the consensus sequence in promoters from Lactococcus lactis. Bellow you can see our promoters and their name in the part registry. • CP1 - BBa_K1033219 • CP8 - BBa_K1033220 • CP11 - BBa_K1033221 • CP29 - BBa_K1033222 • CP30 - BBa_K1033223 • CP41 - BBa_K1033224 • CP44 - BBa_K1033225
