Team:Goettingen/Project/OurProject

From 2013.igem.org

(Difference between revisions)
(→Our Porject)
m (→Our Porject)
Line 39: Line 39:
<p>Our project is aimed at the development of  a simple screening system, which allows the rapid identification and characterization of substances that disturb c-di-AMP homeostasis in pathogenic bacteria. </p>
<p>Our project is aimed at the development of  a simple screening system, which allows the rapid identification and characterization of substances that disturb c-di-AMP homeostasis in pathogenic bacteria. </p>
<p> The principle of how such a screening  system could look like is illustrated in Figure 1.  The screening system will be established in the  non-pathogenic bacterium E. coli. </p>
<p> The principle of how such a screening  system could look like is illustrated in Figure 1.  The screening system will be established in the  non-pathogenic bacterium E. coli. </p>
-
<p> First, we want to construct a promoter-reporter gene fusion which allows us to monitor  the activity of a transcription factor that only binds to a specific DNA sequence (operator) in the presence of c-di-AMP. The operator sequence will be placed between a constitutively  active promoter and a reporter gene, such as  lacZ  and  gfp  encoding the -galactosidase and  the green-fluorescent protein (GFP), respectively. </p>
+
<p> First, we want to construct a promoter-reporter gene fusion which allows us to monitor  the activity of a transcription factor that only binds to a specific DNA sequence (operator) in the presence of c-di-AMP. The operator sequence will be placed between a constitutively  active promoter and a reporter gene, such as  lacZ  and  gfp  encoding the -galactosidase and  the green-fluorescent protein (GFP), respectively. For details,please click <a href="/Team:Goettingen/Team/Reporters" class="moreinfo">Reporter Team</a></p>
<p>  The activity of either of the two proteins is very easy to detect. Then, we will evaluate  whether binding of the transcription factor and  thus inhibition of the promoter-reporter gene  fusion can be controlled by exogenous c-di-AMP. </p>
<p>  The activity of either of the two proteins is very easy to detect. Then, we will evaluate  whether binding of the transcription factor and  thus inhibition of the promoter-reporter gene  fusion can be controlled by exogenous c-di-AMP. </p>
<p> Finally, we want to express a diadenylate cyclase  from  B. subtilis  to inhibit the promoter reporter gene fusion by endogenously synthesized c-di-AMP. There are two big advantages of using E. coli as a host for the development of a screening system to  identify antibacterial compounds that interfere  with c-di-AMP homoeostasis in Gram-positive pathogenic bacteria. </p>
<p> Finally, we want to express a diadenylate cyclase  from  B. subtilis  to inhibit the promoter reporter gene fusion by endogenously synthesized c-di-AMP. There are two big advantages of using E. coli as a host for the development of a screening system to  identify antibacterial compounds that interfere  with c-di-AMP homoeostasis in Gram-positive pathogenic bacteria. </p>
Line 45: Line 45:
<p>  We are confident that our screening system will facilitate the identification of novel  antibacterial substances because any change  in the activity of the c-di-AMP-dependent  promoter-reporter gene fusion, either by inhibition of c-di-AMP synthesis or by activation  of DNA-binding activity of the transcription  factor  will indicate perturbation of c-di-AMP homeostasis.  </p>
<p>  We are confident that our screening system will facilitate the identification of novel  antibacterial substances because any change  in the activity of the c-di-AMP-dependent  promoter-reporter gene fusion, either by inhibition of c-di-AMP synthesis or by activation  of DNA-binding activity of the transcription  factor  will indicate perturbation of c-di-AMP homeostasis.  </p>
</html>
</html>
 +
======previous next======
======previous next======
<html>
<html>

Revision as of 20:17, 26 June 2013

The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria


Our Porject

Our project:

Our project is aimed at the development of a simple screening system, which allows the rapid identification and characterization of substances that disturb c-di-AMP homeostasis in pathogenic bacteria.

The principle of how such a screening system could look like is illustrated in Figure 1. The screening system will be established in the non-pathogenic bacterium E. coli.

First, we want to construct a promoter-reporter gene fusion which allows us to monitor the activity of a transcription factor that only binds to a specific DNA sequence (operator) in the presence of c-di-AMP. The operator sequence will be placed between a constitutively active promoter and a reporter gene, such as lacZ and gfp encoding the -galactosidase and the green-fluorescent protein (GFP), respectively. For details,please click Reporter Team

The activity of either of the two proteins is very easy to detect. Then, we will evaluate whether binding of the transcription factor and thus inhibition of the promoter-reporter gene fusion can be controlled by exogenous c-di-AMP.

Finally, we want to express a diadenylate cyclase from B. subtilis to inhibit the promoter reporter gene fusion by endogenously synthesized c-di-AMP. There are two big advantages of using E. coli as a host for the development of a screening system to identify antibacterial compounds that interfere with c-di-AMP homoeostasis in Gram-positive pathogenic bacteria.

First, c-di-AMP is not synthesized in E. coli. Thus, compounds that inhibit c-di-AMP synthesis will specifically inhibit growth of Gram-positive bacteria. Second, the use of a nonpathogenic E. coli strain, which is easy to cultivate will keep the costs very low.

We are confident that our screening system will facilitate the identification of novel antibacterial substances because any change in the activity of the c-di-AMP-dependent promoter-reporter gene fusion, either by inhibition of c-di-AMP synthesis or by activation of DNA-binding activity of the transcription factor will indicate perturbation of c-di-AMP homeostasis.

previous next

Previous Next



Retrieved from "http://2013.igem.org/Team:Goettingen/Project/OurProject"