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Team:Goettingen/Team/Array
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<p>By using microarray analysis we would like to determine, which genes are regulated by ci-di-AMP. In order to determine this, we are using three different Bacillus subtilus strains. The first strain contains all three active diadenylatecyclase disA, cdaA, cdaS. The second strain is mutant strain (ΔdisA). The third strain is a double mutant strain, which only contains one active diadenylatecyclase (ΔdisA, ΔcdaA). The microarray analysis will then show, which genes are expressed under different intracellular ci-di-AMP concentrations. Furthermore, we would like to create a triple knock-out mutant (ΔdisA, ΔcdaA, ΔcdaS). We are trying to create this triple knock-out mutant via a feeding experiment. </p> | <p>By using microarray analysis we would like to determine, which genes are regulated by ci-di-AMP. In order to determine this, we are using three different Bacillus subtilus strains. The first strain contains all three active diadenylatecyclase disA, cdaA, cdaS. The second strain is mutant strain (ΔdisA). The third strain is a double mutant strain, which only contains one active diadenylatecyclase (ΔdisA, ΔcdaA). The microarray analysis will then show, which genes are expressed under different intracellular ci-di-AMP concentrations. Furthermore, we would like to create a triple knock-out mutant (ΔdisA, ΔcdaA, ΔcdaS). We are trying to create this triple knock-out mutant via a feeding experiment. </p> | ||
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Revision as of 21:20, 27 June 2013
