Team:SUSTC-Shenzhen-A/Notebook

From 2013.igem.org

(Difference between revisions)

Revision as of 22:34, 27 September 2013

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Protocol and notes	Safety and security	Attributions	Human Practice

July
M	T	W	T	F	S	S
[http://2013.igem.org/wiki/index.php?title=SUSTC/1_July_2013&action=edit 1]	[http://2013.igem.org/wiki/index.php?title=SUSTC/2_July_2013&action=edit 2]	[http://2013.igem.org/wiki/index.php?title=SUSTC/3_July_2013&action=edit 3]	[http://2013.igem.org/wiki/index.php?title=SUSTC/4_July_2013&action=edit 4]	[http://2013.igem.org/wiki/index.php?title=SUSTC/5_July_2013&action=edit 5]	[http://2013.igem.org/wiki/index.php?title=SUSTC/6_July_2013&action=edit 6]	[http://2013.igem.org/wiki/index.php?title=SUSTC/7_July_2013&action=edit 7]
[http://2013.igem.org/wiki/index.php?title=SUSTC/8_July_2013&action=edit 8]	[http://2013.igem.org/wiki/index.php?title=SUSTC/9_July_2013&action=edit 9]	[http://2013.igem.org/wiki/index.php?title=SUSTC/10_July_2013&action=edit 10]	[http://2013.igem.org/wiki/index.php?title=SUSTC/11_July_2013&action=edit 11]	[http://2013.igem.org/wiki/index.php?title=SUSTC/12_July_2013&action=edit 12]	[http://2013.igem.org/wiki/index.php?title=SUSTC/13_July_2013&action=edit 13]	[http://2013.igem.org/wiki/index.php?title=SUSTC/14_July_2013&action=edit 14]
[http://2013.igem.org/wiki/index.php?title=SUSTC/15_July_2013&action=edit 15]	[http://2013.igem.org/wiki/index.php?title=SUSTC/16_July_2013&action=edit 16]	[http://2013.igem.org/wiki/index.php?title=SUSTC/17_July_2013&action=edit 17]	[http://2013.igem.org/SUSTC/18_July_2013 18]	[http://2013.igem.org/SUSTC/19_July_2013 19]	[http://2013.igem.org/wiki/index.php?title=SUSTC/20_July_2013&action=edit 20]	[http://2013.igem.org/wiki/index.php?title=SUSTC/21_July_2013&action=edit 21]
[http://2013.igem.org/SUSTC/22_July_2013 22]	[http://2013.igem.org/wiki/index.php?title=SUSTC/23_July_2013&action=edit 23]	[http://2013.igem.org/wiki/index.php?title=SUSTC/24_July_2013&action=edit 24]	[http://2013.igem.org/SUSTC/25_July_2013 25]	[http://2013.igem.org/wiki/index.php?title=SUSTC/26_July_2013&action=edit 26]	[http://2013.igem.org/wiki/index.php?title=SUSTC/27_July_2013&action=edit 27]	[http://2013.igem.org/SUSTC/28_July_2013 28]
[http://2013.igem.org/wiki/index.php?title=SUSTC/29_July_2013&action=edit 29]	[http://2013.igem.org/wiki/index.php?title=SUSTC/30_July_2013&action=edit 30]	[http://2013.igem.org/wiki/index.php?title=SUSTC/31_July_2013&action=edit 31]

August
M	T	W	T	F	S	S
			[http://2013.igem.org/wiki/index.php?title=SUSTC/1_August_2013&action=edit 1]	[http://2013.igem.org/wiki/index.php?title=SUSTC/2_August_2013&action=edit 2]	[http://2013.igem.org/wiki/index.php?title=SUSTC/3_August_2013&action=edit 3]	[http://2013.igem.org/wiki/index.php?title=SUSTC/4_August_2013&action=edit 4]
[http://2013.igem.org/wiki/index.php?title=SUSTC/5_August_2013&action=edit 5]	[http://2013.igem.org/wiki/index.php?title=SUSTC/6_August_2013&action=edit 6]	[http://2013.igem.org/wiki/index.php?title=SUSTC/7_August_2013&action=edit 7]	[http://2013.igem.org/wiki/index.php?title=SUSTC/8_August_2013&action=edit 8]	[http://2013.igem.org/SUSTC/9_August_2013 9]	[http://2013.igem.org/SUSTC/10_August_2013 10]	[http://2013.igem.org/wiki/index.php?title=SUSTC/11_August_2013&action=edit 11]
[http://2013.igem.org/SUSTC/12_August_2013 12]	[http://2013.igem.org/SUSTC/13_August_2013 13]	[http://2013.igem.org/SUSTC/14_August_2013 14]	[http://2013.igem.org/SUSTC/15_August_2013 15]	[http://2013.igem.org/SUSTC/16_August_2013 16]	[http://2013.igem.org/SUSTC/17_August_2013 17]	[http://2013.igem.org/SUSTC/18_August_2013 18]
[http://2013.igem.org/SUSTC/19_August_2013 19]	[http://2013.igem.org/SUSTC/20_August_2013 20]	[http://2013.igem.org/SUSTC/21_August_2013 21]	[http://2013.igem.org/SUSTC/22_August_2013 22]	[http://2013.igem.org/wiki/index.php?title=SUSTC/23_August_2013&action=edit 23]	[http://2013.igem.org/SUSTC/24_August_2013 24]	[http://2013.igem.org/SUSTC/25_August_2013 25]
[http://2013.igem.org/SUSTC/26_August_2013 26]	[http://2013.igem.org/SUSTC/27_August_2013 27]	[http://2013.igem.org/SUSTC/28_August_2013 28]	[http://2013.igem.org/SUSTC/29_August_2013 29]	[http://2013.igem.org/SUSTC/30_August_2013 30]	[http://2013.igem.org/SUSTC/31_August_2013 31]

September
M	T	W	T	F	S	S
						[http://2013.igem.org/SUSTC/1_September_2013 1]
[http://2013.igem.org/wiki/index.php?title=SUSTC/2_September_2013&action=edit 2]	[http://2013.igem.org/SUSTC/3_September_2013 3]	[http://2013.igem.org/SUSTC/4_September_2013 4]	[http://2013.igem.org/SUSTC/5_September_2013 5]	[http://2013.igem.org/wiki/index.php?title=SUSTC/6_September_2013&action=edit 6]	[http://2013.igem.org/SUSTC/7_September_2013 7]	[http://2013.igem.org/SUSTC/8_September_2013 8]
[http://2013.igem.org/SUSTC/9_September_2013 9]	[http://2013.igem.org/SUSTC/10_September_2013 10]	[http://2013.igem.org/SUSTC/11_September_2013 11]	[http://2013.igem.org/SUSTC/12_September_2013 12]	[http://2013.igem.org/SUSTC/13_September_2013 13]	[http://2013.igem.org/SUSTC/14_September_2013 14]	[http://2013.igem.org/SUSTC/15_September_2013 15]
[http://2013.igem.org/SUSTC/16_September_2013 16]	[http://2013.igem.org/SUSTC/17_September_2013 17]	[http://2013.igem.org/wiki/index.php?title=SUSTC/18_September_2013&action=edit 18]	[http://2013.igem.org/wiki/index.php?title=SUSTC/19_September_2013&action=edit 19]	[http://2013.igem.org/wiki/index.php?title=SUSTC/20_September_2013&action=edit 20]	[http://2013.igem.org/wiki/index.php?title=SUSTC/21_September_2013&action=edit 21]	[http://2013.igem.org/wiki/index.php?title=SUSTC/22_September_2013&action=edit 22]
[http://2013.igem.org/wiki/index.php?title=SUSTC/23_September_2013&action=edit 23]	[http://2013.igem.org/wiki/index.php?title=SUSTC/24_September_2013&action=edit 24]	[http://2013.igem.org/wiki/index.php?title=SUSTC/25_September_2013&action=edit 25]	[http://2013.igem.org/wiki/index.php?title=SUSTC/26_September_2013&action=edit 26]	[http://2013.igem.org/wiki/index.php?title=SUSTC/27_September_2013&action=edit 27]	[http://2013.igem.org/wiki/index.php?title=SUSTC/28_September_2013&action=edit 28]	[http://2013.igem.org/wiki/index.php?title=SUSTC/29_September_2013&action=edit 29]
[http://2013.igem.org/wiki/index.php?title=SUSTC/30_September_2013&action=edit 30]

Notebook

We started our project in middle July. You can find every detail by clicking the date.

18th July to 10th Aug: Training

12th Aug to 17th Sep: Project and biobricks.

Protocol

         Preparation of LB culture
         Preparation of some antibiotic solution
         Transformation
         Preservation of the bacteria
         Extract the target DNA
         The use of spectrometer
         Usage of PCR-Cycler
         Usage of ultraviolet spectrophotometer
         Preparation for the M9 culture
         Preparation of the nitrate LB culture
         Usage of FCM
         Preparation of the gel
               Recycle of the DNA in the gel and deal with gel
                             The recycle of the products of the PCR
                            The use of the microplate reader
                            Check he function of the xylose and the nitrate inducible promote

@@ Line 50: / Line 50: @@
 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Preparation of the gel">Preparation of the gel</a><br>
 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Recycle of the DNA in the gel and deal with gel"> Recycle of the DNA in the gel and deal with gel</a><br>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.The recycle of the products of the PCR">The recycle of the products of the PCR</a><br>
+&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.The recycle of the products of the PCR">The recycle of the products of the PCR</a><br>
 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.The use of the microplate reader">The use of the microplate reader</a><br>
 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index. check the function of the xylose and the nitrate inducible promote">Check he function of the xylose and the nitrate inducible promote</a><br>
 </html>