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Team:CSU Fort Collins/SHItires
From 2013.igem.org
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So before we even attempted to engineer new yeast, we needed an initial trial to see if the tires would even inflate with hydrogen peroxide and yeast. We used a 26" bicycle tire as our testing apparatus. To measure pressure we used a Fluke Multimeter to directly measure millivolts from a pressure transducer hooked up to the bicycle tire. We combined the yeast (20ml), 10% H2O2 (100ml), YPD (130ml) in the tire itself sequentially. Once mixed, we immediately attached the pressure transducer and began recording data once every 10 seconds. Below is the graph of our results. | So before we even attempted to engineer new yeast, we needed an initial trial to see if the tires would even inflate with hydrogen peroxide and yeast. We used a 26" bicycle tire as our testing apparatus. To measure pressure we used a Fluke Multimeter to directly measure millivolts from a pressure transducer hooked up to the bicycle tire. We combined the yeast (20ml), 10% H2O2 (100ml), YPD (130ml) in the tire itself sequentially. Once mixed, we immediately attached the pressure transducer and began recording data once every 10 seconds. Below is the graph of our results. | ||
<img src="https://static.igem.org/mediawiki/2013/9/9f/Tire_Graph_CSUFC_2013.png" alt="Tire Inflation Graph"> | <img src="https://static.igem.org/mediawiki/2013/9/9f/Tire_Graph_CSUFC_2013.png" alt="Tire Inflation Graph"> | ||
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Revision as of 23:20, 27 September 2013
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Bike Tire Inflation and Puncture Repair
Edit pageHere's the Idea
Results
So before we even attempted to engineer new yeast, we needed an initial trial to see if the tires would even inflate with hydrogen peroxide and yeast. We used a 26" bicycle tire as our testing apparatus. To measure pressure we used a Fluke Multimeter to directly measure millivolts from a pressure transducer hooked up to the bicycle tire. We combined the yeast (20ml), 10% H2O2 (100ml), YPD (130ml) in the tire itself sequentially. Once mixed, we immediately attached the pressure transducer and began recording data once every 10 seconds. Below is the graph of our results.
