Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/5.6 Phage From Lysate

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Latest revision as of 00:24, 28 September 2013


Phage Purification May - June Notebook: Experiments



Phage Purification
March-April
May-June
July-August
September-October

5.6 Phage From Lysate


I) Purpose

Separate Phage from bacterial lysate to store viable phage for extended periods of time.

II) Expected Outcome

Viable phage, easily stored.

III) Materials Used

2 mL eppendorf tubes (Qty 20)
5 mL cultures of BL 21 (infected with T7 phage for 24 hours) and W3110 (infected with T4 phage for 7 hours)


IV) Actual Procedure

Separated the 5 mL cultures into 5 eppendorfs, 1 mL in each.
Centrifuged at 3050 rpm for 5 min
Gently extracted the supernatant and placed into clean eppendorf tubes.

V) Results

The phage were still viable and are stable when stored in the fridge.


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