Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation

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Latest revision as of 00:29, 28 September 2013


Phage Purification May - June Notebook: Experiments



Phage Purification
March-April
May-June
July-August
September-October

6.7 Phage Suspension Buffer Purification


I) Purpose

Create 1 L of 10X Phage Supension Buffer stock.

II) Expected Outcome

Clean, usable stock of phage suspension buffer.

III) Reagants Used

NaCl
MgCl2
Tris-HCl

IV) Actual Procedure

Put 12.4 g Tris-HCl in a 1 L flask. Added 1 L H2O. Titrated to 7.2 pH using 50% NaOH. (.1 M Tris-HCl)
Added 58.44 g NaCl (1M)
Added 9.52 g MgCl2 (.1M)
Autoclaved and stored in a sealed bottle for use.

V) Results

Created 1 L of 10X phage suspension buffer


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