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Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Explist
From 2013.igem.org
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This procedure purifies mutated T7 phage from the small phage team. | This procedure purifies mutated T7 phage from the small phage team. | ||
| - | Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.76CsClGradient|9.7 CsCl Gradient]] | + | : Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.76CsClGradient|9.7 CsCl Gradient]] |
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We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients. | We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients. | ||
| - | : | + | : Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]] |
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- We ran another T7 phage purification procedure with new PSB. | - We ran another T7 phage purification procedure with new PSB. | ||
| - | : | + | : Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]] |
Revision as of 00:41, 28 September 2013
| Phage Purification August - September Notebook: September 1 - September 14 Experiment Listing
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9.7 CsCl Gradient This procedure purifies mutated T7 phage from the small phage team.
9.9 10X Phage Suspension Buffer Creation We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients.
9/11/13 - We ran another T7 phage purification procedure with new PSB.
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