Team:KAIT Japan/Results

From 2013.igem.org

(Difference between revisions)
Line 29: Line 29:
:*Figure2 transformation of Il10recepter β  
:*Figure2 transformation of Il10recepter β  
<big>2 .culture on the LB nutrient medium and get colonies.</big>
<big>2 .culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>3 colony PCR</big>
<big>3 colony PCR</big>
:The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp
:The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp
Line 36: Line 37:
‎<br>
‎<br>
:*Figure4 Il10recepter β colony PCR
:*Figure4 Il10recepter β colony PCR
 +
<br>
<big>4 restriction enzyme processing PCR production</big>
<big>4 restriction enzyme processing PCR production</big>
 +
<br>
<big>5 infusion</big>
<big>5 infusion</big>
 +
<br>
<big>6 .transformation</big>
<big>6 .transformation</big>
 +
<br>
<big>7 culture on the LB nutrient medium and get colonies.</big>
<big>7 culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>8 colony PCR</big>
<big>8 colony PCR</big>
:The molecular weight of the vector is a 4000 ~ 5000bp.
:The molecular weight of the vector is a 4000 ~ 5000bp.
Line 46: Line 52:
<big>'''2 STAT3'''</big>
<big>'''2 STAT3'''</big>
:We inserted stat3 gene into vector
:We inserted stat3 gene into vector
 +
<br>
<big>1 transformation</big>
<big>1 transformation</big>
<br>
<br>
Line 51: Line 58:
:*Figure5 transformation of STAT3  
:*Figure5 transformation of STAT3  
<big>2 .culture on the LB nutrient medium and get colonies.</big>
<big>2 .culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>3 colony PCR</big>
<big>3 colony PCR</big>
:The molecular weight of the α chain is 2307bp.
:The molecular weight of the α chain is 2307bp.
[[File:Fig6.png]]
[[File:Fig6.png]]
:*Figure6 STAT3 colony PCR  
:*Figure6 STAT3 colony PCR  
 +
<br>
<big>4 restriction enzyme processing PCR production</big>
<big>4 restriction enzyme processing PCR production</big>
 +
<br>
<big>5 infusion</big>
<big>5 infusion</big>
 +
<br>
<big>6 .transformation</big>
<big>6 .transformation</big>
 +
<br>
<big>7 culture on the LB nutrient medium and get colonies.</big>
<big>7 culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>8 colony PCR
<big>8 colony PCR
:The molecular weight of the vector is a 4000 ~ 5000bp.
:The molecular weight of the vector is a 4000 ~ 5000bp.
Line 65: Line 78:
<big>'''3 GFP+HlyA'''</big>
<big>'''3 GFP+HlyA'''</big>
:We inserted GFP gene and HlyA into vecter  
:We inserted GFP gene and HlyA into vecter  
 +
<br>
<big>1 transformation</big>
<big>1 transformation</big>
<br>
<br>
Line 71: Line 85:
:*Figure7 transformation of HlyA.
:*Figure7 transformation of HlyA.
<big>2 .culture on the LB nutrient medium and get colonies.</big>
<big>2 .culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>3 colony PCR</big>
<big>3 colony PCR</big>
:The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp
:The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp
Line 76: Line 91:
:*Figure8 Il-10recepter GFP PCR
:*Figure8 Il-10recepter GFP PCR
:*Figure9 Il-10recepter HlyA PCR
:*Figure9 Il-10recepter HlyA PCR
 +
<br>
<big>4 restriction enzyme processing PCR production</big>
<big>4 restriction enzyme processing PCR production</big>
 +
<br>
<big>5 infusion</big>
<big>5 infusion</big>
 +
<br>
<big>6 .transformation</big>
<big>6 .transformation</big>
 +
<br>
<big>7 culture on the LB nutrient medium and get colonies.</big>
<big>7 culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>8 colony PCR</big>
<big>8 colony PCR</big>
:The molecular weight of the vector is a 2000~3000bp.
:The molecular weight of the vector is a 2000~3000bp.

Revision as of 03:49, 28 September 2013

KAIT Japan 2013RogoTop.png

KAIT Japan2013 Kanako.png KAIT Japan 2013 iGEMRogo.png

KAIT Japan home.png Home
KAIT Japan team.png Team
KAIT Japan project.png Project
KAIT Japan parts.png Parts
KAIT Japan protocol.png Protocol
KAIT Japan notebook.png Notebook

KAIT_Japan_results.png Results

KAIT Japan safety.png Safety
KAIT Japan human practice.png Human Practice


Results

1.IL10recepter

We inserted Il10receptere gene into vecter

1 transformation
Figure1.jpeg

  • Figure 1transformation of Il10recepter α

Figure2.jpeg

  • Figure2 transformation of Il10recepter β

2 .culture on the LB nutrient medium and get colonies.
3 colony PCR

The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp

Fig1.png

  • Figure3 Il10recepter α colony PCR

Fig2.png ‎

  • Figure4 Il10recepter β colony PCR


4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR

The molecular weight of the vector is a 4000 ~ 5000bp.
We were not able to confirm the vector by colony PCR.


2 STAT3

We inserted stat3 gene into vector


1 transformation
Figure5.jpeg

  • Figure5 transformation of STAT3

2 .culture on the LB nutrient medium and get colonies.
3 colony PCR

The molecular weight of the α chain is 2307bp.

Fig6.png

  • Figure6 STAT3 colony PCR


4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR

The molecular weight of the vector is a 4000 ~ 5000bp.
We were not able to confirm the vector by colony PCR.


3 GFP+HlyA

We inserted GFP gene and HlyA into vecter


1 transformation
Figure7.jpeg

  • Figure7 transformation of HlyA.

2 .culture on the LB nutrient medium and get colonies.
3 colony PCR

The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp

HlyA1.jpg

  • Figure8 Il-10recepter GFP PCR
  • Figure9 Il-10recepter HlyA PCR


4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR

The molecular weight of the vector is a 2000~3000bp.
We were not able to confirm the vector by colony PCR.






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