"
Team:IIT Delhi/Project
From 2013.igem.org
(Difference between revisions)
| Line 229: | Line 229: | ||
The idea is to create a biological device that can utilize the continuous nature of transcriptional regulation (that is a more accurate representation of reality as opposed to discrete circuits) and give a gradient response. pH based response systems have not been taken up extensively in iGEM as such and offer fertile grounds for research in this field of study. A primary reason for this can be that most of the biosensors are designed to facilitate maximum growth. However, pH sensing is based on challenging this very concept.<br><br> | The idea is to create a biological device that can utilize the continuous nature of transcriptional regulation (that is a more accurate representation of reality as opposed to discrete circuits) and give a gradient response. pH based response systems have not been taken up extensively in iGEM as such and offer fertile grounds for research in this field of study. A primary reason for this can be that most of the biosensors are designed to facilitate maximum growth. However, pH sensing is based on challenging this very concept.<br><br> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/2/24/Phcoli.png" align= right height= 400 width = | + | <img src="https://static.igem.org/mediawiki/2013/2/24/Phcoli.png" align= right height= 400 width = 350> |
<br<br> | <br<br> | ||
Our idea is to combine the studies exploring pH inducible promoters in molecular biology with logical systems present in the iGEM parts registry to create a Global Bacterial pH sensor akin to the Universal pH Indicator. We have made a mathematical model of our circuit and simulated it on MATLAB to observe the response characteristics. We have also indicated through a simple wet lab experiment that RFP degradation is independent of pH. | Our idea is to combine the studies exploring pH inducible promoters in molecular biology with logical systems present in the iGEM parts registry to create a Global Bacterial pH sensor akin to the Universal pH Indicator. We have made a mathematical model of our circuit and simulated it on MATLAB to observe the response characteristics. We have also indicated through a simple wet lab experiment that RFP degradation is independent of pH. | ||
Revision as of 04:14, 28 September 2013
 |
| Making a Comprehensive Bacterial pH sensor The idea is to create a biological device that can utilize the continuous nature of transcriptional regulation (that is a more accurate representation of reality as opposed to discrete circuits) and give a gradient response. pH based response systems have not been taken up extensively in iGEM as such and offer fertile grounds for research in this field of study. A primary reason for this can be that most of the biosensors are designed to facilitate maximum growth. However, pH sensing is based on challenging this very concept. 
|
Feel Free to contact us at igemiitdelhi2013 at gmail dot com if you have queries; requests; suggestions et cetera. Thanks to iGEM and IIT Delhi, we had an awesome summer! |
Our
Project was supported by and done by the students of IIT Delhi, India.  |
This
project was done as a part of iGEM: iGEM Main Website  |
