Team:UNITN-Trento/Extra/Judging

• Register the team, have a great summer, and plan to have fun at the Regional Jamboree.
• Successfully complete and submit the iGEM 2013 Judging form.
• Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.
• Plan to present a Poster and Talk at the iGEM Jamboree.
• Document at least one new standard BioBrick Part or Device used in the project/central to your project and submit this part to the iGEM Registry.
  We have constructed and documented 14 new Parts helpful for the creation of a photo inducible fruit ripening control circuit, but not only. These are our entries:
  BBa_K1065000, BBa_K1065001, BBa_K1065002, BBa_K1065101, BBa_K1065102, BBa_K1065104, BBa_K1065105, BBa_K1065106, BBa_K1065203, BBa_K1065204, BBa_K1065306, BBa_K1065308, BBa_K1065307, BBa_K1065309, BBa_K1065311.

• Experimentally validate that at least one new BioBrick Part or Device of your own design and construction work as expected.
  Our Best Biobrick BBa_K1065002: the Ethylene Forming Enzyme (EFE) was shown to produce ethylene and ripen all of the fruit that we tested!
• Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry Submit this new part to the iGEM Parts Registry. We characterized this part in two different ways:
  • under the control of the arabinose inducible promoter;
  • under the control of a blue light device.
• Describe in the wiki one or more ways in which the environment, security, safety and ethics and/or ownership and sharing or other broader implications have been taken into consideration in the design and execution of your project.
  We have thoroughly analysed the possible pathways available in nature for ethylene production. The choice of the right pathway was done keeping always in mind safety and environmental issues. For example, we decided not to use the plants ethylene pathway, which would produce also cyanide...! Check our Safety page for specific info.

• Improve the function of an existing BioBrick Part or Device, enter this information in the 'Experience' section of that Parts's Registry entry, create a new registry page for the improved part, and submit this part to the iGEM Registry.
  We have improved 1 existing BioBricks from the registry: Bba_K592003.
  Our new entries for this part are:
  • Bba_K1065303 (new entry for Bba_K592003);
  • Bba_K1065305 (new entry for Bba_K592003).
• We also have resubmitted in pSB1C3 two parts from the Eau de coli MIT 2006 project that were discontinued. We hope that these parts will be included in the 2014 registry and will be soon available to many other teams.
  Our entries for these parts are:
  • BBa_K1065100 (new entry for BBa_J45119);
  • BBa_K1065103 (new entry for BBa_J45319).
• Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.

We have designed an innovative way to provide the consumer with ready-to-eat fruit that exploits our engineered bacterial system. For more details visit the Vending Machine page.