Team:Goettingen/Team/Array

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===Array Team===
===Array Team===
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<p>By using microarray analysis we would like to determine, which genes are regulated by ci-di-AMP. In order to determine this, we are using three different Bacillus subtilus strains. The first strain contains all three active diadenylatecyclase disA, cdaA, cdaS. The second strain is mutant strain (<i>ΔdisA</i>). The third strain is a double mutant strain, which only contains one active diadenylatecyclase (<i>ΔdisA, ΔcdaA</i>). The microarray analysis will then show, which genes are expressed under different intracellular ci-di-AMP concentrations. Furthermore, we would like to create a triple knock-out mutant (<i>ΔdisA, ΔcdaA, ΔcdaS</i>). We are trying to create this triple knock-out mutant via a feeding experiment. </p>
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<p>By using the microarray technique we would like to analyze how many genes are regulated by ci-di-AMP in the Gram-positive model bacterium <i>Bacillus subtilis</i>. We are not only interested in the impact of c-di-AMP on the transcriptome, we also hope to identify novel regulatory elements (i.e. riboswitches) that bind to the signaling molecule. In our experiments we use three different <i>B. subtilis</i> strains. The wild-type strain contains all three diadenylatecyclases DisA, CdaA, CdaS. The second strain is a <i>disA</i> mutant strain lacking DisA, which was shown to be involved in DNA metabolism. This strain should produce less c-di-AMP than the isogenic parent strain. The third strain synthesizes a hyperactive CdaS mutant variant, which produces a lot of c-di-AMP. In our microarray experiments we will compare the transcriptomes of each mutant strain with that of the wild-type strain. Moreover, we would like to create a triple knock-out mutant (<i>ΔdisA, ΔcdaA, ΔcdaS</i>) lacking all diadenylate cyclases. We try to construct the triple knock-out mutant by feeding with exogenous c-di-AMP. </p>
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Revision as of 17:54, 29 September 2013





The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria



Array Team

By using the microarray technique we would like to analyze how many genes are regulated by ci-di-AMP in the Gram-positive model bacterium Bacillus subtilis. We are not only interested in the impact of c-di-AMP on the transcriptome, we also hope to identify novel regulatory elements (i.e. riboswitches) that bind to the signaling molecule. In our experiments we use three different B. subtilis strains. The wild-type strain contains all three diadenylatecyclases DisA, CdaA, CdaS. The second strain is a disA mutant strain lacking DisA, which was shown to be involved in DNA metabolism. This strain should produce less c-di-AMP than the isogenic parent strain. The third strain synthesizes a hyperactive CdaS mutant variant, which produces a lot of c-di-AMP. In our microarray experiments we will compare the transcriptomes of each mutant strain with that of the wild-type strain. Moreover, we would like to create a triple knock-out mutant (ΔdisA, ΔcdaA, ΔcdaS) lacking all diadenylate cyclases. We try to construct the triple knock-out mutant by feeding with exogenous c-di-AMP.

 

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