Team:UNITN-Trento/Project/Bacillus
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Bacillus Subtilis | Bacillus Subtilis | ||
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+ | When we first came up with the idea of B- fruity, we immediatly thought that b.subtilis was the perfect chassis for a possible marketable application: | ||
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+ | <i>Bacillus subtilis</i> sporulates and it can be stored in a inactive state; | ||
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+ | <i>Bacillus subtilis</i> is not pathogenic; | ||
+ | </li> | ||
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+ | we have always worked only with <i>E. coli</i> and we thought to try out how it is to employ a new organism. It’s been really challenging but we got it! | ||
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+ | </ol> | ||
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+ | <i>Bacillus subtilis</i> would be the perfect chassis for a fruit-ripening household product, that exploit ethylene (or MeSA) production upon spores activation. We have designed a <i>B. fruity</i> home edition that exploits this principle. <br/> | ||
+ | To achieve this goal we started working with EFE, a ethylene forming enzyme from <i>Pseudomas Syringae</i> pv. phaseolicola (<a href="http://parts.igem.org/Part:BBa_K1065002">BBa_K1065002</a>), which were inserted into pSBBs0K-Pspac (IPGT inducible) and pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for <i>B. subtilis</i> by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich). | ||
+ | <img src="https://static.igem.org/mediawiki/2013/8/85/Tn-2013-project_ethylene-BBa_K1065001.jpg"> | ||
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Revision as of 08:34, 30 September 2013
Bacillus Subtilis
When we first came up with the idea of B- fruity, we immediatly thought that b.subtilis was the perfect chassis for a possible marketable application:
To achieve this goal we started working with EFE, a ethylene forming enzyme from Pseudomas Syringae pv. phaseolicola (BBa_K1065002), which were inserted into pSBBs0K-Pspac (IPGT inducible) and pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for B. subtilis by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich).
- Bacillus subtilis sporulates and it can be stored in a inactive state;
- Bacillus subtilis is not pathogenic;
- we have always worked only with E. coli and we thought to try out how it is to employ a new organism. It’s been really challenging but we got it!
To achieve this goal we started working with EFE, a ethylene forming enzyme from Pseudomas Syringae pv. phaseolicola (BBa_K1065002), which were inserted into pSBBs0K-Pspac (IPGT inducible) and pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for B. subtilis by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich).
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