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Team:Paris Bettencourt/Notebook/Trojan Horse/Wednesday 17th July.html
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Latest revision as of 20:06, 3 October 2013
Trojan Horse
ASDF17th July
Anne
Redsign of Primers
Design primers to PCR KanR out of Duet and LacZalpha out of pUC18 to then clone them into the DUET containing Chloramphenicol
Geneious files in the dropbox: LacZ primers, KanR primers
Clovis and Anne
Liquid Cultures (for Miniprep)
7ml LB with proper antibiotics
adding: 1) NEB with pUC18 (AmpR)
2) sT002 : MG1655 pCOLADuet-1 (KanR)
3) sT003 : MG1655 pACYCDuet-1 (ChlaR)
Cultivate over night in the 37°C shaker
Vincent
Check: GFP expressed as expected in ELS-41
Lauching O/N of ELS-41 and ELS-13 with Amp and Kan, respectively.
Aude
Lauching O/N of MGZ1 (Glycerol from lab upstairs) with Spec (500X)
